College of Horticulture, Northeast Agricultural University, Harbin 150030, China.
Int J Mol Sci. 2022 Jun 25;23(13):7078. doi: 10.3390/ijms23137078.
Tomato crown and root rot (FCRR) is an extremely destructive soil-borne disease. To date, studies have shown that only plants with tomato mosaic virus (TMV) resistance exhibit similar resistance to tomato f. sp. (FORL) and have identified a single relevant gene, , in Peruvian tomato. Due to the relative lack of research on FCRR disease-resistance genes in China and elsewhere, transcriptome data for FORL-resistant (cv. '19912') and FORL-susceptible (cv. 'Moneymaker') tomato cultivars were analysed for the first time in this study. The number of differentially expressed genes (DEGs) was higher in Moneymaker than in 19912, and 189 DEGs in the 'plant-pathogen interaction' pathway were subjected to GO and KEGG enrichment analyses. and genes were enriched in major metabolic pathways related to plant disease resistance; thus, we focused on these two gene families. In the early stage of tomato infection, the content of JA and SA increased, but the change in JA was more obvious. Fourteen genes were selected for confirmation of their differential expression levels by qRT-PCR. This study provides a series of novel disease resistance resources for tomato breeding and genetic resources for screening and cloning FORL resistance genes.
番茄冠根腐病（FCRR）是一种极具破坏性的土传病害。迄今为止的研究表明，只有具有抗番茄花叶病毒（TMV）特性的植物才对番茄黄化曲叶病毒（FORL）表现出相似的抗性，并在秘鲁番茄中鉴定出一个单一的相关基因 。由于在中国和其他地方对 FCRR 抗病基因的研究相对较少，本研究首次对 FORL 抗性（cv. '19912'）和 FORL 敏感（cv. 'Moneymaker'）番茄品种的转录组数据进行了分析。Moneymaker 中的差异表达基因（DEGs）数量高于 19912，在“植物-病原体相互作用”途径中对 189 个 DEGs 进行了 GO 和 KEGG 富集分析。 和 基因在与植物抗病性相关的主要代谢途径中得到了富集；因此，我们专注于这两个基因家族。在番茄感染的早期阶段，JA 和 SA 的含量增加，但 JA 的变化更为明显。通过 qRT-PCR 对 14 个基因进行了差异表达水平的验证。本研究为番茄抗病育种提供了一系列新的抗病资源，为筛选和克隆 FORL 抗病基因提供了遗传资源。
