酿酒酵母细胞壁突变体与野生型中β-葡聚糖结构的比较。

Comparison of beta-glucan structures in a cell wall mutant of Saccharomyces cerevisiae and the wild type.

作者信息

Shiota M, Nakajima T, Satoh A, Shida M, Matsuda K

出版信息

J Biochem. 1985 Nov;98(5):1301-7. doi: 10.1093/oxfordjournals.jbchem.a135397.

DOI:10.1093/oxfordjournals.jbchem.a135397

Abstract

A mutant of Saccharomyces cerevisiae defective in the cell wall beta-glucan structure was obtained. The mutant cells are extremely sensitive to (beta 1-3)-glucanase digestion and mild alkali treatment. Structural analysis revealed that the alkali-insoluble, skeletal glucan from wild type cells contains two components, a (beta 1-3) linked glucan with a laminated structure, and a highly branched glucan containing predominantly (beta 1-6) linkages. The mutant cells lack the latter component.

摘要

获得了一种细胞壁β-葡聚糖结构有缺陷的酿酒酵母突变体。该突变体细胞对（β1-3）-葡聚糖酶消化和温和碱处理极为敏感。结构分析表明，野生型细胞的碱不溶性骨架葡聚糖包含两种成分，一种具有层状结构的（β1-3）连接的葡聚糖，以及一种主要含有（β1-6）连接的高度分支的葡聚糖。突变体细胞缺乏后一种成分。