Fleet G H, Manners D J
J Gen Microbiol. 1977 Feb;98(2):315-27. doi: 10.1099/00221287-98-2-315.
An alkali-soluble glucan from the cell walls of Saccharomyces cerevisiae NCYC1109 has been hydrolysed with a purified endo-(1 leads to 3)-beta-D-glucanase and an endo-(1 leads to 6)-beta-D-glucanase from Bacillus circulans WL-12. The products of enzyme action include various oligosaccharide and polysaccharide fractions which have been separated by gel filtration and characterized, giving new information on the fine structure of the glucan. The isolated cell walls have also been subjected to enzymic hydrolysis. The results suggest that part of the cell-wall mannan is held in place by a glucan component.
来自酿酒酵母NCYC1109细胞壁的一种碱溶性葡聚糖，已被来自环状芽孢杆菌WL-12的纯化内切-(1→3)-β-D-葡聚糖酶和内切-(1→6)-β-D-葡聚糖酶水解。酶作用的产物包括通过凝胶过滤分离并表征的各种寡糖和多糖级分，为葡聚糖的精细结构提供了新信息。分离出的细胞壁也进行了酶水解。结果表明，部分细胞壁甘露聚糖由葡聚糖成分固定在原位。
