College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China.
Department of Nutrition and Health, China Agricultural University, Beijing 100193, China.
Food Chem. 2024 Dec 1;460(Pt 3):140679. doi: 10.1016/j.foodchem.2024.140679. Epub 2024 Jul 31.
This study aimed to reveal the change patterns of the phosphorylation modification status of yak whey phosphoproteins during lactation and their physiological effects. Herein, we comprehensively characterized whey phosphoproteome in yak colostrum and mature milk using an ultra-high throughput phosphoproteomics approach incorporating trapped ion mobility technology. A total of 344 phosphorylation sites from 206 phosphoproteins were identified, with individual site modification predominating. Notably, 117 significantly different phosphorylation sites were distributed on 89 whey phosphoproteins. Gene ontology analysis indicated that these significantly different whey phosphoproteins (SDWPPs) were mainly annotated to carbohydrate metabolic process, membrane, extracellular region and calcium ion binding. Metabolic pathway enrichment analysis demonstrated that SDWPPs were critically involved in protein processing in endoplasmic reticulum, NOD-like receptor signaling pathway and N-glycan biosynthesis. Our results elucidate the phosphorylation profiles of yak whey phosphoproteins at different lactations and their adaptive regulatory role in meeting the nutritional requirements of yak calves during development.
本研究旨在揭示牦牛乳清磷酸化蛋白在泌乳期的磷酸化修饰状态变化模式及其生理作用。为此，我们采用包含离子淌度技术的超高通量磷酸化蛋白质组学方法，全面表征了牦牛初乳和成熟乳中的乳清磷酸蛋白质组。共鉴定到 206 种乳清磷酸蛋白中的 344 个磷酸化位点，以单个位点修饰为主。值得注意的是，89 种乳清磷酸蛋白上分布着 117 个差异显著的磷酸化位点。GO 分析表明，这些差异显著的乳清磷酸蛋白（SDWPP）主要注释到碳水化合物代谢过程、膜、细胞外区域和钙离子结合。代谢途径富集分析表明，SDWPPs 主要参与内质网中蛋白质加工、NOD 样受体信号通路和 N-糖生物合成。本研究阐明了不同泌乳期牦牛乳清磷酸化蛋白的磷酸化谱及其在满足牦牛幼仔发育过程中营养需求的适应性调节作用。
