National Corn to Ethanol Research Center at Southern Illinois University Edwardsville, 400 University Park Dr, Edwardsville, IL 62025, USA.
National Corn to Ethanol Research Center at Southern Illinois University Edwardsville, 400 University Park Dr, Edwardsville, IL 62025, USA.
Bioresour Technol. 2024 Sep;408:131216. doi: 10.1016/j.biortech.2024.131216. Epub 2024 Aug 4.
Fractionated corn bran was processed to maximize ethanol production from starch, cellulose, and xylan. After various bench-scale experiments, an optimized process with dilute acid pretreatment (1.5 % w/w HSO) at 90 °C for 60 min was utilized followed by enzymatic hydrolysis using cellulase and hemicellulase for 48 hr. After simultaneous saccharification (regarding starch) and fermentation at 150 L using an engineered yeast, which consumes both glucose and xylose to make ethanol, the 86 % total sugar conversion yield was achieved, including conversions of 95 % for starch, 77 % for cellulose and 77 % for xylan. Also, an accurate mass balance was formulated for ethanol-producing carbohydrates including starch, cellulose, and xylan from feedstock to final ethanol. A highly efficient process of converting corn fiber to ethanol was successfully scaled up to 150 L.
分馏玉米麸皮经加工后，可最大限度地从淀粉、纤维素和木聚糖中提取乙醇。经过各种小规模实验，采用了优化的工艺，即用 1.5%（w/w）HSO 在 90°C 下预处理 60 分钟，然后用纤维素酶和半纤维素酶进行 48 小时的酶水解。在使用既能消耗葡萄糖又能消耗木糖来生产乙醇的工程酵母在 150 L 下进行同步糖化和发酵后，实现了 86%的总糖转化率，其中淀粉转化率为 95%，纤维素转化率为 77%，木聚糖转化率为 77%。此外，还制定了从原料到最终乙醇的生产碳水化合物（包括淀粉、纤维素和木聚糖）的精确质量平衡。成功地将玉米纤维高效转化为乙醇的工艺扩大到了 150 L。
