Graduate School of Science and Technology, Kumamoto University, Kumamoto City, Kumamoto, Japan.
Prep Biochem Biotechnol. 2013;43(7):682-95. doi: 10.1080/10826068.2013.773338.
Corn stover is the most abundant agricultural residue in China and a valuable reservoir for bioethanol production. In this study, we proposed a process for producing bioethanol from corn stover; the pretreatment prior to presaccharification, followed by simultaneous saccharification and fermentation (SSF) by using a flocculating Saccharomyces cerevisiae strain, was optimized. Pretreatment with acid-alkali combination (1% H2SO4, 150 °C, 10 min, followed by 1% NaOH, 80°C, 60 min) resulted in efficient lignin removal and excellent recovery of xylose and glucose. A glucose recovery efficiency of 92.3% was obtained by enzymatic saccharification, when the pretreated solid load was 15%. SSF was carried out at 35 °C for 36 hr after presaccharification at 50 °C for 24 hr, and an ethanol yield of 88.2% was achieved at a solid load of 15% and an enzyme dosage of 15 FPU/g pretreated corn stover.
玉米秸秆是中国最丰富的农业废弃物，也是生产生物乙醇的有价值的资源库。在本研究中，我们提出了一种从玉米秸秆生产生物乙醇的工艺；采用絮凝酿酒酵母菌株，对预处理前的预糖化、同步糖化和发酵（SSF）进行了优化。酸-碱联合预处理（1%H2SO4，150°C，10 分钟，然后用 1%NaOH，80°C，60 分钟）可有效去除木质素，并可极好地回收木糖和葡萄糖。当预处理的固体负荷为 15%时，酶解可获得 92.3%的葡萄糖回收率。SSF 在预糖化 50°C 24 小时后在 35°C 下进行 36 小时，在 15%的固体负荷和 15 FPU/g 预处理玉米秸秆的酶剂量下，可实现 88.2%的乙醇得率。
