从引起苹果叶斑病的植物病原菌链格孢中分离到的一种新型杆状病毒的全基因组序列。
Complete genome sequence of a novel mitovirus isolated from the phytopathogenic fungus Alternaria alternata causing apple leaf blotch.
机构信息
College of Plant Protection, Shanxi Agricultural University, Taigu, 030801, Shanxi, China.
出版信息
Arch Virol. 2024 Aug 6;169(9):174. doi: 10.1007/s00705-024-06106-3.
In this study, a novel mitovirus, tentatively designated as "Alternaria alternata mitovirus 2" (AaMV2), was isolated from the fungus Alternaria alternata f. sp. mali causing apple leaf blotch disease. The complete genome of AaMV2 is 3,157 nucleotides in length, with an A+U content of 68.10%. The genome has a single large open reading frame (ORF) encoding an RNA-dependent RNA polymerase (RdRp) protein with a molecular mass of 98.10 kDa. BLAST analysis revealed that AaMV2 has the highest sequence identity to Leptosphaeria biglobosa mitovirus 6, with 79.76% and 82.86% identity at the amino acid and nucleotide level, respectively. Phylogenetic analysis suggested that AaMV2 is a new member of the genus Duamitovirus within the family Mitoviridae. This is the first report of the complete genome sequence analysis of a mitovirus in A. alternata.
在这项研究中，一种新型的呼肠孤病毒，暂定名为“链格孢菌呼肠孤病毒 2 型”（AaMV2），从引起苹果叶斑病的真菌链格孢Alternaria alternata f. sp. mali 中分离得到。AaMV2 的完整基因组长 3157 个核苷酸，A+U 含量为 68.10%。基因组具有一个单一的大开放阅读框（ORF），编码一个分子量为 98.10 kDa 的 RNA 依赖性 RNA 聚合酶（RdRp）蛋白。BLAST 分析表明，AaMV2 与大丽轮枝菌呼肠孤病毒 6 具有最高的序列同一性，在氨基酸和核苷酸水平上的同一性分别为 79.76%和 82.86%。系统发育分析表明，AaMV2 是呼肠孤病毒科 Duamitovirus 属的一个新成员。这是首次报道链格孢菌呼肠孤病毒的全基因组序列分析。
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