College of Forestry, Central South University of Forestry and Technology, Changsha, China.
College of Materials Science and Engineering, Central South University of Forestry and Technology, Changsha, China.
Arch Virol. 2024 Aug 16;169(9):181. doi: 10.1007/s00705-024-06110-7.
Here, we characterized a novel mitovirus from the fungus Nigrospora oryzae, which was named "Nigrospora oryzae mitovirus 3" (NoMV3). The NoMV3 genome is 2,492 nt in length with a G + C content of 33%, containing a single large open reading frame (ORF) using the fungal mitochondrial genetic code. The ORF encodes an RNA-dependent RNA polymerase (RdRp) of 775 amino acids with a molecular mass of 88.75 kDa. BLASTp analysis revealed that the RdRp of NoMV3 had 68.6%, 50.6%, and 48.6% sequence identity to those of Nigrospora oryzae mitovirus 2, Suillus luteus mitovirus 6, and Fusarium proliferatum mitovirus 3, respectively, which belong to the genus Unuamitovirus within the family Mitoviridae. Phylogenetic analysis based on amino acid sequences supported the classification of NoMV3 as a member of a new species in the genus Unuamitovirus within the family Mitoviridae.
这里，我们对来自真菌稻曲病菌的一种新型杆状病毒进行了描述，将其命名为“稻曲病菌杆状病毒 3 号”（NoMV3）。NoMV3 基因组长 2492 个核苷酸，G+C 含量为 33%，包含一个使用真菌线粒体遗传密码的单一大型开放阅读框（ORF）。ORF 编码一个 775 个氨基酸的 RNA 依赖性 RNA 聚合酶（RdRp），分子量为 88.75kDa。BLASTp 分析显示，NoMV3 的 RdRp 与 Nigrospora oryzae mitovirus 2、Suillus luteus mitovirus 6 和 Fusarium proliferatum mitovirus 3 的 RdRp 分别具有 68.6%、50.6%和 48.6%的序列同一性，它们均属于杆状病毒科 Unuamitovirus 属。基于氨基酸序列的系统发育分析支持将 NoMV3 分类为杆状病毒科 Unuamitovirus 属的一个新种。
