Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Nongda Road 1, Furong District, Changsha, 410128, Hunan, People's Republic of China.
Agricultural Science Institute of XiangXi Tujia and Miao Autonomous Prefecture, Xiangxi, 416000, People's Republic of China.
Arch Virol. 2021 May;166(5):1507-1511. doi: 10.1007/s00705-021-05023-z. Epub 2021 Mar 8.
In this study, we isolated and determined the complete genome sequence of a novel mitovirus, "Botryosphaeria dothidea mitovirus 2" (BdMV2), from the phytopathogenic fungus Botryosphaeria dothidea isolate DT-5. BdMV2 has a genome 2,482 nt in length with an A+U content of 67%. The genome of BdMV2 contains a single large open reading frame (ORF) encoding an RNA-dependent RNA polymerase (RdRp) of 717 amino acids (aa) with a molecular mass of 81.86 kDa. A BLASTp comparison of the RdRp sequence showed the highest identity (66.67%) with that of Alternaria arborescens mitovirus 1 (AbMV1). Sequence comparisons and phylogenetic analysis revealed that BdMV2 is a new member of the genus Mitovirus of the family Mitoviridae.
在这项研究中，我们从病原菌 Botryosphaeria dothidea 分离株 DT-5 中分离并确定了一种新型的呼肠孤病毒，即“BdMV2”（Botryosphaeria dothidea mitovirus 2）的完整基因组序列。BdMV2 的基因组长度为 2482 个核苷酸，A+U 含量为 67%。BdMV2 的基因组包含一个单一的大开放阅读框（ORF），编码一个 717 个氨基酸（aa）的 RNA 依赖性 RNA 聚合酶（RdRp），分子量为 81.86 kDa。RdRp 序列的 BLASTp 比较显示，与 Alternaria arborescens mitovirus 1（AbMV1）的同源性最高（66.67%）。序列比较和系统发育分析表明，BdMV2 是 Mitoviridae 科 Mitovirus 属的一个新成员。
