Department of Oncology, Tongde Hospital of Zhejiang, affiliated to Zhejiang Chinese Medicine University, Hangzhou, China.
Department of Respiratory, Jinhua Guangfu Hospital, Jinhua, China.
Clin Respir J. 2024 Aug;18(8):e13812. doi: 10.1111/crj.13812.
Qi deficiency and phlegm dampness (QPD) is one of the most common traditional Chinese medicine (TCM) syndromes in lung adenocarcinoma (LUAD). This study aimed to identify syndrome-specific biomarkers for LUAD with QPD syndrome.
Peripheral blood mononuclear cells (PBMCs) from LUAD patients with QPD, LUAD patients with non-QPD (N-QPD), and healthy control (H) were collected and analyzed with RNA-seq to identify differentially expressed genes (DEGs). The area under the receiver operator characteristic curve (AUC) of each DEG was calculated, and the top 10 highest AUC DEGs were validated by qRT-PCR. Logistic regression analysis was used to develop a diagnostic model evaluated with AUC.
A total of 135 individuals were enrolled in this study (training set: 15 QPD, 15 N-QPD, 15 H; validation set: 30 QPD, 30 N-QPD, 30 H). A total of 1480 DEGs were identified between QPD and N-QPD. The qRT-PCR results showed that the expression of DDR2 was downregulated, and PPARG was upregulated, which was in line with the finding of the training set. We developed a diagnostic model with these two genes. The AUC of the diagnostic model in the training cohort and validation cohort was 0.891 and 0.777, respectively.
We identified the two genes (DDR2 and PPARG) as syndrome-specific biomarkers for LUAD with QPD syndrome and developed a novel diagnostic model, which may help to improve the accuracy and sensibility of clinical diagnosis and provide a new target for natural drug treatment of LUAD.
气阴两虚夹痰证（QPD）是肺腺癌（LUAD）中最常见的中医（TCM）证候之一。本研究旨在鉴定 LUAD 伴 QPD 证候的证候特异性生物标志物。
收集 LUAD 伴 QPD、LUAD 不伴 QPD（N-QPD）和健康对照（H）患者的外周血单核细胞（PBMCs），并进行 RNA-seq 分析以鉴定差异表达基因（DEGs）。计算每个 DEG 的受试者工作特征曲线下面积（AUC），并通过 qRT-PCR 验证 top10 个 AUC 最高的 DEGs。采用逻辑回归分析构建 AUC 评估的诊断模型。
本研究共纳入 135 名患者（训练集：15 例 QPD、15 例 N-QPD、15 例 H；验证集：30 例 QPD、30 例 N-QPD、30 例 H）。QPD 与 N-QPD 之间共鉴定出 1480 个 DEGs。qRT-PCR 结果显示 DDR2 表达下调，PPARG 表达上调，与训练集结果一致。我们基于这两个基因构建了一个诊断模型。该诊断模型在训练集和验证集的 AUC 分别为 0.891 和 0.777。
我们鉴定出 DDR2 和 PPARG 这两个基因作为 LUAD 伴 QPD 证候的证候特异性生物标志物，并构建了一个新的诊断模型，这可能有助于提高临床诊断的准确性和敏感性，并为 LUAD 的天然药物治疗提供新的靶点。
