Pinna Nicola, Ben Abbou Salwa, Ianni Federica, Angeles Flores Giancarlo, Pietercelie Anne, Perretti Giuseppe, Blasi Francesca, Angelini Paola, Cossignani Lina
Department of Pharmaceutical Sciences, University of Perugia, 06126 Perugia, Italy.
Department of Sciences and Techniques, Institut Meurice, 1070 Bruxelles, Belgium.
Food Chem X. 2024 Jul 6;23:101628. doi: 10.1016/j.fochx.2024.101628. eCollection 2024 Oct 30.
Ultrasound-assisted extraction of (poly)phenols from pumpkin pulp was optimized using response surface methodology. Time, solvent-to-sample ratio and percentage of water in ethanol were considered and a full factorial design was applied. The optimized conditions (10 min; 30 mL/g; 50% water in ethanol) were used to isolate (poly)phenols from five pumpkin varieties (Hokkaido, Lunga di Napoli, Mantovana, Moscata di Provenza, Violina rugosa), collected for two successive years. Phenolic acids were the most abundant phenolic class (gallic acid up to 413.50 μg/g), even if differences were observed among varieties and harvesting years. Generally, Violina rugosa harvested in 2021 showed the highest total phenol content (7.59 mg gallic acid equivalents/g) and antioxidant properties (16.93 mg Trolox equivalents/g). All pumpkin variety exhibited antimicrobial activity within a concentration range of 1.95 to 250 μg/mL. In conclusion, the pumpkin pulp can be considered a promising natural source of (poly)phenols for the development of health-promoting products.
采用响应面法对南瓜果肉中（多）酚类物质的超声辅助提取工艺进行了优化。考虑了提取时间、溶剂与样品的比例以及乙醇中的含水量，并采用了全因子设计。利用优化条件（10分钟；30毫升/克；乙醇中50%的水）从连续两年收集的五个南瓜品种（北海道、那不勒斯长南瓜、曼托瓦纳南瓜、普罗旺斯摩斯卡塔南瓜、皱叶小提琴南瓜）中分离（多）酚类物质。酚酸是含量最丰富的酚类类别（没食子酸含量高达413.50微克/克），不过不同品种和收获年份之间存在差异。总体而言，2021年收获的皱叶小提琴南瓜总酚含量最高（7.59毫克没食子酸当量/克），抗氧化性能也最强（16.93毫克 Trolox 当量/克）。所有南瓜品种在1.95至250微克/毫升的浓度范围内均表现出抗菌活性。总之，南瓜果肉可被视为开发促进健康产品的一种有前景的天然（多）酚类物质来源。
