Matzkin Luciano M, Bono Jeremy M, Pigage Helen K, Allan Carson W, Diaz Fernando, McCoy John R, Green Clinton C, Callan Jeffrey B, Delahunt Stephen P
Department of Entomology, University of Arizona, Tucson, AZ, USA.
BIO5 Institute, University of Arizona, Tucson, AZ, USA.
iScience. 2024 Jul 2;27(8):110442. doi: 10.1016/j.isci.2024.110442. eCollection 2024 Aug 16.
Although RNA is found in the seminal fluid of diverse organisms, it is unknown whether it is functional within females. We developed a proteomic method (VESPA, Variant Enabled SILAC Proteomic Analysis) to test the hypothesis that male seminal fluid RNA is translated by females. We found 67 male-derived, female-translated proteins (mdFTPs) in female lower reproductive tracts, many with predicted functions relevant to reproduction. Knockout experiments indicate that mdFTPs play diverse roles in postmating interactions, affecting fertilization success, and the formation/persistence of the insemination reaction mass, a trait hypothesized to be involved in sexual conflict. These findings advance our understanding of reproduction by revealing a mechanism of postmating molecular interactions between the sexes that strengthens and extends male influences on reproduction in previously unrecognized ways. Given the diverse species that carry RNA in seminal fluid, this discovery has broad significance for understanding molecular mechanisms of cooperation and conflict during reproduction.
尽管在多种生物的精液中都发现了RNA，但尚不清楚它在雌性体内是否具有功能。我们开发了一种蛋白质组学方法（VESPA，即变异体启用的稳定同位素标记蛋白质组分析）来检验雄性精液RNA是否能被雌性翻译这一假设。我们在雌性下生殖道中发现了67种雄性来源、雌性翻译的蛋白质（mdFTPs），其中许多具有与生殖相关的预测功能。基因敲除实验表明，mdFTPs在交配后的相互作用中发挥着多种作用，影响受精成功率以及授精反应物质的形成/持续存在，而授精反应物质这一特征被认为与性冲突有关。这些发现揭示了一种两性之间交配后分子相互作用的机制，以之前未被认识的方式加强并扩展了雄性对生殖的影响，从而增进了我们对生殖的理解。鉴于精液中携带RNA的物种多种多样，这一发现对于理解生殖过程中合作与冲突的分子机制具有广泛意义。
