• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

蛙皮素-BO1通过HSP90A-Cdc37-CDK1轴诱导肝癌细胞周期停滞和凋亡。

Bombinin-BO1 induces hepatocellular carcinoma cell-cycle arrest and apoptosis via the HSP90A-Cdc37-CDK1 axis.

作者信息

Wang Xiaoting, Tang Peiyu, Gong Yitong, Yao Hang, Liang Mingpeng, Qu Hongxia, Han Xiao, Zheng Qiusheng, Shi Zhen, Li Defang, Jiang Qingling

机构信息

Yantai Key Laboratory of Pharmacology of Traditional Chinese Medicine in Tumor Metabolism, School of Traditional Chinese Medicine, Binzhou Medical University, Yantai, Shandong 264003, P.R. China.

Collaborative Innovation Platform for Modernization and Industrialization of Regional Characteristic Traditional Chinese Medicine, School of Traditional Chinese Medicine, Binzhou Medical University, Yantai, Shandong 264003, P.R. China.

出版信息

iScience. 2024 Jun 26;27(8):110382. doi: 10.1016/j.isci.2024.110382. eCollection 2024 Aug 16.

DOI:10.1016/j.isci.2024.110382
PMID:39108730
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11301096/
Abstract

Bombinin-BO1 (BO1), a bombinin peptide derived from the skin secretion of , exhibits broad-spectrum antimicrobial activity. To date, the anticancer effect of BO1 remains unclear. This study confirmed cytotoxicity of BO1 on hepatocellular carcinoma cells by inducing S-phase cycle block and apoptosis. In addition, BO1 was found to be localized in cytoplasm through endocytosis. The combined results of pull down, mass spectrometry, and co-immunoprecipitation suggested that BO1 induced misfolding of CDK1 and degradation by competitively binding HSP90A with Cdc37. It was verified that overexpression of HSP90A in BO1-treated cells significantly inhibited degradation of CDK1. , BO1 inhibited tumor without being toxic to individuals. This study reveals the anti-tumor mechanism of BO1 in inducing cell-cycle arrest and apoptosis by interfering with HSP90A-Cdc37-CDK1 system. This is the first study to analyze the mechanism of BO1 regulation of tumor cells, providing theoretical basis for BO1 treatment of hepatocellular carcinoma.

摘要

蛙皮素-BO1（BO1）是一种源自蛙皮分泌物的蛙皮素肽，具有广谱抗菌活性。迄今为止，BO1的抗癌作用仍不清楚。本研究通过诱导S期周期阻滞和细胞凋亡证实了BO1对肝癌细胞的细胞毒性。此外，发现BO1通过内吞作用定位于细胞质中。下拉实验、质谱分析和免疫共沉淀的综合结果表明，BO1通过与Cdc37竞争性结合HSP90A诱导CDK1错误折叠并降解。证实BO1处理细胞中HSP90A的过表达显著抑制了CDK1的降解。此外，BO1抑制肿瘤且对个体无毒。本研究揭示了BO1通过干扰HSP90A-Cdc37-CDK1系统诱导细胞周期阻滞和细胞凋亡的抗肿瘤机制。这是第一项分析BO1调节肿瘤细胞机制的研究，为BO1治疗肝癌提供了理论依据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd6f/11301096/5f93175e0060/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd6f/11301096/5936d70f43a2/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd6f/11301096/e37ed1b4649d/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd6f/11301096/ab2e5129fa1e/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd6f/11301096/48b46e0ce134/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd6f/11301096/93115e2f1330/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd6f/11301096/d2990ade4fec/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd6f/11301096/dadead09d25a/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd6f/11301096/739a506b7e28/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd6f/11301096/5f93175e0060/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd6f/11301096/5936d70f43a2/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd6f/11301096/e37ed1b4649d/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd6f/11301096/ab2e5129fa1e/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd6f/11301096/48b46e0ce134/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd6f/11301096/93115e2f1330/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd6f/11301096/d2990ade4fec/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd6f/11301096/dadead09d25a/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd6f/11301096/739a506b7e28/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd6f/11301096/5f93175e0060/gr8.jpg

相似文献

1
Bombinin-BO1 induces hepatocellular carcinoma cell-cycle arrest and apoptosis via the HSP90A-Cdc37-CDK1 axis.蛙皮素-BO1通过HSP90A-Cdc37-CDK1轴诱导肝癌细胞周期停滞和凋亡。
iScience. 2024 Jun 26;27(8):110382. doi: 10.1016/j.isci.2024.110382. eCollection 2024 Aug 16.
2
Molecular characterization and bioactivity evaluation of two novel bombinin peptides from the skin secretion of Oriental fire-bellied toad, Bombina orientalis.从东方铃蟾皮肤分泌物中分离得到两种新型蛙皮素肽的分子特征和生物活性评价。
Amino Acids. 2018 Feb;50(2):241-253. doi: 10.1007/s00726-017-2509-z. Epub 2017 Nov 2.
3
Feleucin-BO1: a novel antimicrobial non-apeptide amide from the skin secretion of the toad, Bombina orientalis, and design of a potent broad-spectrum synthetic analogue, feleucin-K3.东方铃蟾抗菌九肽酰胺Feleucin-BO1：一种从东方铃蟾皮肤分泌物中提取的新型抗菌九肽酰胺，以及强效广谱合成类似物Feleucin-K3的设计
Chem Biol Drug Des. 2015 Mar;85(3):259-67. doi: 10.1111/cbdd.12396. Epub 2014 Aug 11.
4
(20S) Ginsenoside Rh2 Exerts Its Anti-Tumor Effect by Disrupting the HSP90A-Cdc37 System in Human Liver Cancer Cells.(20S)人参皂苷Rh2通过破坏人肝癌细胞中的HSP90A-Cdc37系统发挥其抗肿瘤作用。
Int J Mol Sci. 2021 Dec 6;22(23):13170. doi: 10.3390/ijms222313170.
5
Discovery of two bombinin peptides with antimicrobial and anticancer activities from the skin secretion of Oriental fire-bellied toad, Bombina orientalis.从东方铃蟾（Bombina orientalis）皮肤分泌物中发现具有抗菌和抗癌活性的两种蛙皮素肽。
Chem Biol Drug Des. 2018 Jan;91(1):50-61. doi: 10.1111/cbdd.13055. Epub 2017 Jul 19.
6
The synergistic antimicrobial effects of novel bombinin and bombinin H peptides from the skin secretion of .新型蛙皮素和蛙皮素 H 肽对 的皮肤分泌物的协同抗菌作用。
Biosci Rep. 2017 Sep 27;37(5). doi: 10.1042/BSR20170967. Print 2017 Oct 31.
7
Suppressing the CDC37 cochaperone in hepatocellular carcinoma cells inhibits cell cycle progression and cell growth.在肝癌细胞中抑制辅伴侣蛋白CDC37会抑制细胞周期进程和细胞生长。
Liver Int. 2015 Apr;35(4):1403-15. doi: 10.1111/liv.12651. Epub 2014 Aug 25.
8
Improved Aitongxiao prescription (I-ATXP) induces apoptosis, cell cycle arrest and blocks exosomes release in hepatocellular carcinoma (HCC) cells.改良艾痛消方（I-ATXP）诱导肝癌（HCC）细胞凋亡、细胞周期阻滞并阻断外泌体释放。
Int J Physiol Pathophysiol Pharmacol. 2022 Apr 15;14(2):90-113. eCollection 2022.
9
Kaempferol from Alpinia officinarum hance induces G2/M cell cycle arrest in hepatocellular carcinoma cells by regulating the ATM/CHEK2/KNL1 pathway.高良姜中的山奈酚通过调节ATM/CHEK2/KNL1通路诱导肝癌细胞发生G2/M期细胞周期阻滞。
J Ethnopharmacol. 2024 Oct 28;333:118430. doi: 10.1016/j.jep.2024.118430. Epub 2024 Jun 8.
10
Metformin induces miR-378 to downregulate the CDK1, leading to suppression of cell proliferation in hepatocellular carcinoma.二甲双胍诱导miR-378下调细胞周期蛋白依赖性激酶1（CDK1），从而抑制肝癌细胞增殖。
Onco Targets Ther. 2018 Jul 31;11:4451-4459. doi: 10.2147/OTT.S167614. eCollection 2018.

本文引用的文献

1
TuBG1 promotes hepatocellular carcinoma via ATR/P53-apoptosis and cycling pathways.微管蛋白γ1通过ATR/P53-凋亡和细胞周期途径促进肝细胞癌。
Hepatobiliary Pancreat Dis Int. 2024 Apr;23(2):195-209. doi: 10.1016/j.hbpd.2023.09.004. Epub 2023 Sep 26.
2
HSP90, as a functional target antigen of a mAb 11C9, promotes stemness and tumor progression in hepatocellular carcinoma.热休克蛋白 90（HSP90）作为单抗 11C9 的功能靶标，促进肝癌的干性和肿瘤进展。
Stem Cell Res Ther. 2023 Sep 27;14(1):273. doi: 10.1186/s13287-023-03453-x.
3
Diagnostic and Prognostic Value of Protein Post-translational Modifications in Hepatocellular Carcinoma.
蛋白质翻译后修饰在肝细胞癌中的诊断和预后价值
J Clin Transl Hepatol. 2023 Oct 28;11(5):1192-1200. doi: 10.14218/JCTH.2022.00006S. Epub 2023 Apr 4.
4
Roles and Molecular Mechanisms of Biomarkers in Hepatocellular Carcinoma with Microvascular Invasion: A Review.具有微血管侵犯的肝细胞癌中生物标志物的作用及分子机制：综述
J Clin Transl Hepatol. 2023 Oct 28;11(5):1170-1183. doi: 10.14218/JCTH.2022.00013S. Epub 2023 May 4.
5
RETRACTED: Hemp bioactive peptides: Nutrition, functional properties and action mechanisms to maximize their nutraceutical applications and future prospects.撤回：大麻生物活性肽：营养、功能特性及作用机制，以最大化其营养保健应用及未来前景
Food Chem. 2023 Jul 15;414:135691. doi: 10.1016/j.foodchem.2023.135691. Epub 2023 Feb 14.
6
Cdc37 as a Co-chaperone to Hsp90.Cdc37 作为 Hsp90 的共伴侣。
Subcell Biochem. 2023;101:141-158. doi: 10.1007/978-3-031-14740-1_5.
7
Recent advances in recombinase polymerase amplification: Principle, advantages, disadvantages and applications.近年来重组酶聚合酶扩增技术的进展：原理、优点、缺点及应用。
Front Cell Infect Microbiol. 2022 Nov 28;12:1019071. doi: 10.3389/fcimb.2022.1019071. eCollection 2022.
8
Wnt/beta-catenin signaling and its modulators in nonalcoholic fatty liver diseases.Wnt/β-catenin 信号通路及其在非酒精性脂肪性肝病中的调节剂。
Hepatobiliary Pancreat Dis Int. 2023 Aug;22(4):333-345. doi: 10.1016/j.hbpd.2022.10.003. Epub 2022 Oct 20.
9
Cancer statistics, 2022.癌症统计数据，2022 年。
CA Cancer J Clin. 2022 Jan;72(1):7-33. doi: 10.3322/caac.21708. Epub 2022 Jan 12.
10
Hsa_circ_0003258 promotes prostate cancer metastasis by complexing with IGF2BP3 and sponging miR-653-5p.hsa_circ_0003258 通过与 IGF2BP3 复合物并海绵吸附 miR-653-5p 促进前列腺癌转移。
Mol Cancer. 2022 Jan 5;21(1):12. doi: 10.1186/s12943-021-01480-x.