Department of Endocrinology Shengjing Hospital of China Medical University, Shenyang 110004, China.
School of Life Science Liaoning University, 66 Chongshan Middle Road, Shenyang 110036, China.
J Diabetes Res. 2024 Jul 30;2024:1386469. doi: 10.1155/2024/1386469. eCollection 2024.
Gestational diabetes mellitus (GDM) is a pregnancy-related diabetic condition that may cause serious complications. However, its pathogenesis remains unclear. Placental damage due to GDM may lead to several health issues that cannot be ignored. Thus, we aimed to identify the mechanisms underlying GDM by screening differentially expressed genes (DEGs) related to vascular endothelial cells in the GDM databases and verify the expression of these DEGs in the placentas of women afflicted by GDM. We used GDM microarray datasets integrated from the Gene Expression Omnibus (GEO) database. Functional annotation and protein-protein interaction (PPI) analyses were used to screen DEGs. Placental tissues from 20 pregnant women with GDM and 20 healthy pregnant women were collected, and differential gene expression in the placental tissues was verified via qRT-PCR, western blotting, and immunofluorescence. Bioinformatics analysis revealed three significant DEGs: , , and . These genes were all predicted to be underexpressed in patients with GDM. The results of qRT-PCR, western blot, and immunofluorescence analyses indicated that SNAIL2 and PAPP-A in the placenta tissue of patients with GDM were significantly underexpressed. However, TGF1 in the placenta tissues of GDM was significantly overexpressed. SNAIL2, TGF1, and PAPP-A may affect the placentas of pregnant women with GDM, warranting further investigation.
妊娠期糖尿病（GDM）是一种与妊娠相关的糖尿病病症，可能会导致严重的并发症。然而，其发病机制尚不清楚。GDM 引起的胎盘损伤可能会导致一些不容忽视的健康问题。因此，我们旨在通过筛选 GDM 数据库中与血管内皮细胞相关的差异表达基因（DEGs）来确定 GDM 的发病机制，并验证这些 DEGs 在 GDM 患者胎盘组织中的表达情况。我们使用了从基因表达综合数据库（GEO）集成的 GDM 微阵列数据集。功能注释和蛋白质-蛋白质相互作用（PPI）分析用于筛选 DEGs。收集了 20 名患有 GDM 的孕妇和 20 名健康孕妇的胎盘组织，并通过 qRT-PCR、western blot 和免疫荧光验证了胎盘组织中的差异基因表达。生物信息学分析显示了三个显著的 DEGs： 、 、和 。这些基因在 GDM 患者中均被预测为低表达。qRT-PCR、western blot 和免疫荧光分析的结果表明，GDM 患者胎盘组织中的 SNAIL2 和 PAPP-A 明显低表达，而 TGF1 在 GDM 胎盘组织中明显过表达。SNAIL2、TGF1 和 PAPP-A 可能会影响患有 GDM 的孕妇的胎盘，值得进一步研究。
