Zhu Ping, Wu Zhaoji, Yang Zhilu, Tang Tingting, Liao Yunhui, Zhao Wen, Huang Ying, Chen Tao, Li Junjie, Nong Chunmei, Wu Zhenzhen, Hu Guodong, Liu Yanshan, Chen Yinghua
Department of Histology and Embryology, NMPA Key Laboratory for Safety Evaluation of Cosmetics, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China.
Dongguan People's Hospital Biobank, Affiliated Dongguan Hospital, Southern Medical University, Dongguan, Guangdong 523059, China.
Research (Wash D C). 2024 Jun 6;7:0344. doi: 10.34133/research.0344. eCollection 2024.
Hyperproliferative keratinocytes and subcutaneous inflammation contribute to the characteristic symptoms of psoriasis, including erythema, scales, or scaly plaques on the skin. These symptoms significantly affect patients' quality of life and cause severe physical and psychological distress. However, current treatment strategies have limited therapeutic effect and may lead to adverse side effects. In this study, we present the novel organic photosensitizer TBTDC [5-(((5-(7-(4-(diphenylamino)phenyl)benzo[c][1,2,5]thiadiazol-4-yl)thiophen-2-yl)methylene)amino)-3-methylthiophene-2,4-dicarbonitrile] nanoparticles (NPs) with aggregation-induced emission (AIE) characteristics to mediate photodynamic therapy (TBTDC NP-PDT) for psoriasis treatment. We demonstrate that TBTDC NPs effectively generate reactive oxygen species upon light irradiation and lead to significant apoptosis of psoriatic keratinocytes. Furthermore, TBTDC NPs exhibit high cellular uptake in diseased keratinocytes and induce endoplasmic reticulum stress (ERS)-mediated autophagy, which can also enhance apoptosis. Importantly, TBTDC NPs show no cytotoxicity toward keratinocytes. These unique properties of TBTDC NPs enable remarkable therapeutic effects against psoriasis-like skin lesions and related inflammation in vivo. Overall, our AIE-active TBTDC NP-PDT represents a promising strategy for treating psoriasis in clinical settings.
角质形成细胞过度增殖和皮下炎症导致了银屑病的特征性症状，包括皮肤红斑、鳞屑或鳞屑斑块。这些症状严重影响患者的生活质量，造成严重的身心痛苦。然而，目前的治疗策略治疗效果有限，且可能导致不良副作用。在本研究中，我们展示了一种具有聚集诱导发光（AIE）特性的新型有机光敏剂TBTDC [5 - ((((5 - (7 - (4 - (二苯胺基)phenyl)苯并[c][1,2,5]噻二唑 - 4 - 基)噻吩 - 2 - 基)亚甲基)氨基)-3 - 甲基噻吩 - 2,4 - 二腈]纳米颗粒（NPs），用于介导光动力疗法（TBTDC NP - PDT）治疗银屑病。我们证明，TBTDC NPs在光照下能有效产生活性氧，并导致银屑病角质形成细胞显著凋亡。此外，TBTDC NPs在病变角质形成细胞中表现出高细胞摄取，并诱导内质网应激（ERS）介导的自噬，这也能增强细胞凋亡。重要的是，TBTDC NPs对角质形成细胞无细胞毒性。TBTDC NPs的这些独特性质使其对体内银屑病样皮肤病变和相关炎症具有显著的治疗效果。总体而言，我们的AIE活性TBTDC NP - PDT代表了一种在临床环境中治疗银屑病的有前景的策略。
