College of Plant Health and Medicine, Engineering Research Center for Precision Pest Management for Fruits and Vegetables of Qingdao, Shandong Engineering Research Center for Environment-Friendly Agricultural Pest Management, Shandong Province Key Laboratory of Applied Mycology, Qingdao Agricultural University, Qingdao 266109, China.
College of Life Sciences, Qingdao Agricultural University, Qingdao 266109, China.
Cell Rep. 2024 Aug 27;43(8):114588. doi: 10.1016/j.celrep.2024.114588. Epub 2024 Aug 6.
Plant diseases caused by fungal pathogens pose a great threat to crop production. Conidiation of fungi is critical for disease epidemics and serves as a promising drug target. Here, we show that deacetylation of the FolTFIIS transcription elongation factor is indispensable for Fusarium oxysporum f. sp. lycopersici (Fol) conidiation. Upon microconidiation, Fol decreases K76 acetylation of FolTFIIS by altering the level of controlling enzymes, allowing for its nuclear translocation by FolIws1. Increased nuclear FolTFIIS enhances the transcription of sporulation-related genes and, consequently, enables microconidia production. Deacetylation of FolTFIIS is also critical for the production of macroconidia and chlamydospores, and its homolog has similar functions in Botrytis cinerea. We identify two FolIws1-targeting chemicals that block the conidiation of Fol and have effective activity against a wide range of pathogenic fungi without harm to the hosts. These findings reveal a conserved mechanism of conidiation regulation and provide candidate agrochemicals for disease management.
真菌病原体引起的植物病害对作物生产构成了巨大威胁。真菌的分生孢子形成对于疾病流行至关重要，是一个有前途的药物靶点。在这里，我们表明，转录延伸因子 FolTFIIS 的去乙酰化对于尖孢镰刀菌番茄专化型（Fol）的分生孢子形成是必不可少的。在小分生孢子形成过程中，Fol 通过改变控制酶的水平降低 FolTFIIS 的 K76 乙酰化，从而允许 FolIws1 将其核易位。增加的核 FolTFIIS 增强了与孢子形成相关基因的转录，从而能够产生小分生孢子。FolTFIIS 的去乙酰化对于大分生孢子和厚垣孢子的产生也是至关重要的，其同源物在 Botrytis cinerea 中也具有类似的功能。我们鉴定了两种靶向 FolIws1 的 Fol 分生孢子形成的化学物质，它们可以阻止 Fol 的分生孢子形成，并对广泛的致病真菌具有有效活性，而对宿主没有伤害。这些发现揭示了一个保守的分生孢子形成调控机制，并为疾病管理提供了候选农用化学品。
