New experiments and models to describe soluble surfactant adsorption above and below the critical micelle concentration.

HYPOTHESIS

Lysopalmitoylphosphatidylcholine (LysoPC) is a soluble single-chain surfactant product of the innate immune system degradation of double-chain phospholipids. LysoPC adsorption to the air-water interface in lung alveoli can be modeled using alveolar-sized bubbles of constant surface area in a capillary pressure microtensiometer to show that adsorption is diffusion limited both below and above the critical micelle concentration (CMC). Above the CMC, a local equilibrium model is proposed in which depletion of the local monomer concentration drives dissociation of micelles in a region near the bubble surface.

EXPERIMENTAL

A capillary pressure microtensiometer in which a feedback loop maintains a constant bubble radius and surface area is used to measure dynamic surface tension during LysoPC adsorption. Direct numerical solution of the spherical diffusion equations, a new three parameter virial equation of state for interface thermodynamics, and a local equilibrium model of micellization above the CMC are used to accurately model the dynamic surface tension experiments both below and above the LysoPC CMC.

FINDINGS

LysoPC adsorption is shown to be diffusion-limited over concentrations ranging from below to well above the CMC, and to be well described by a local equilibrium model at concentrations above the CMC. Modelling the dynamic surface tension provides a reliable estimate of the micelle diffusivity near the CMC that is difficult to obtain by other methods in systems with low CMCs.