State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou 730000, China; Guangdong Provincial Research Center for Environment Pollution Control and Remediation Materials, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.
State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou 730000, China; Institute of Marine Science, University of Auckland, Auckland, 1010, New Zealand.
Sci Total Environ. 2024 Nov 1;949:175275. doi: 10.1016/j.scitotenv.2024.175275. Epub 2024 Aug 5.
The gut microbiome plays a crucial role in maintaining animal health and is influenced by various factors, including light exposure; however, the response in laying hens of the gut microbiome to intermittent light regimes and the related impact on antibiotic resistance genes (ARGs) remain poorly understood. In this study, we divided 20-week-old laying hens into two groups. These groups were exposed to either continuous normal light or intermittent light for 8 weeks. The feces and cecal contents of laying hens were collected for analysis. Metagenomic analysis of both feces and cecal content samples revealed significant shifts in the microbial composition and abundance of ARGs under intermittent light exposure compared to normal light exposure (P < 0.05). Furthermore, metabolomic analysis of the cecal contents revealed substantial alterations in the abundance and composition of ARGs and mobile genetic elements (MGEs) in response to intermittent light exposure (P < 0.05). Network analysis revealed intricate co-occurrence patterns among bacterial communities, metabolites, and ARGs, highlighting correlations between Bacteroidetes species, ARGs, and metabolites. Although certain bacterial species showed differential associations, the dominant bacteria carrying ARGs or MGEs had relatively low numbers, suggesting that other bacterial communities may have had a greater influence on ARG dissemination. Moreover, our observations highlight the crucial role of metabolites as mediators between bacterial communities and ARGs, providing novel insights into the dynamics of antibiotic resistance development. Our findings underscore the impact of intermittent light exposure on ARG proliferation in poultry farming and emphasize interconnections among ARGs, bacterial communities, and metabolic pathways. The results underscore the importance of considering both microbial communities and metabolic processes to understand antibiotic resistance in agricultural settings.
肠道微生物群在维持动物健康方面起着至关重要的作用，受到各种因素的影响，包括光照；然而，间歇性光照对蛋鸡肠道微生物群的反应以及对抗生素抗性基因（ARGs）的相关影响仍知之甚少。在这项研究中，我们将 20 周龄的蛋鸡分为两组。这些组分别暴露于连续正常光照或间歇性光照 8 周。收集蛋鸡的粪便和盲肠内容物进行分析。粪便和盲肠内容物样本的宏基因组分析显示，与正常光照相比，间歇性光照下微生物组成和 ARG 丰度发生了显著变化（P<0.05）。此外，盲肠内容物的代谢组学分析表明，间歇性光照下 ARG 和可移动遗传元件（MGEs）的丰度和组成发生了实质性变化（P<0.05）。网络分析显示细菌群落、代谢物和 ARG 之间存在复杂的共现模式，突出了拟杆菌门物种、ARG 和代谢物之间的相关性。尽管某些细菌物种表现出不同的关联，但携带 ARG 或 MGE 的优势细菌数量相对较少，这表明其他细菌群落可能对 ARG 的传播有更大的影响。此外，我们的观察结果强调了代谢物作为细菌群落和 ARG 之间的介质的重要性，为抗生素抗性发展的动态提供了新的见解。我们的研究结果强调了间歇性光照对家禽养殖中 ARG 增殖的影响，并强调了 ARGs、细菌群落和代谢途径之间的相互联系。结果强调了在农业环境中考虑微生物群落和代谢过程来理解抗生素抗性的重要性。
