Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science, South China Agricultural University, Guangzhou 510640, China.
School of Biological Engineering, Henan University of Technology, Zhengzhou 450001, China.
Poult Sci. 2024 Oct;103(10):104138. doi: 10.1016/j.psj.2024.104138. Epub 2024 Jul 29.
Intestinal microbial metabolism has an important impact on the health of laying hens, and microbes are also important hosts for ARGs. However, the relationship between intestinal microbes and antibiotic resistance in laying hens is unclear. In this study, a slaughtering experiment, an in vitro fermentation experiment and a single-bacteria culture experiment were carried out, and metagenomic and metabolomic analyses were used to investigate the relationships between microbial metabolism and the antibiotic resistome in the cecum of laying hens. The results showed that there were different types of ARGs in the intestines of laying hens, and the risk scores of the ARGs tended to decrease with growth stage. A total of 1142 metagenome-assembled genomes (MAGs) were obtained, and Escherichia coli was found to be the dominant ARG host, carrying 62 ARGs. Metabolomics revealed that indole and its derivatives, such as indole-3-lactic acid, were negatively correlated with a variety of ARGs. Moreover, in vitro fermentation experiment and single-bacteria culture experiment demonstrated that indole-3-lactic acid reduced the abundance and risk of multiple ARGs in the intestine and inhibited the growth of the ARG host Escherichia coli. In the context of high concern about intestinal microbial metabolism and antibiotic resistance, this is the first study to focus on the relationship between intestinal microbial metabolism and antibiotic resistance in laying hens. These findings have important implications for healthy farming and antibiotic resistance control.
肠道微生物代谢对蛋鸡的健康有重要影响，微生物也是抗生素抗性基因（ARGs）的重要宿主。然而，肠道微生物与蛋鸡抗生素抗性之间的关系尚不清楚。本研究通过屠宰实验、体外发酵实验和单细菌培养实验，结合宏基因组和代谢组学分析，研究了蛋鸡盲肠中微生物代谢与抗生素抗性组之间的关系。结果表明，蛋鸡肠道中存在不同类型的 ARGs，且 ARGs 的风险评分随生长阶段呈下降趋势。共获得 1142 个宏基因组组装基因组（MAG），发现大肠杆菌是主要的 ARG 宿主，携带 62 个 ARGs。代谢组学分析表明，吲哚及其衍生物（如吲哚-3-乳酸）与多种 ARGs 呈负相关。此外，体外发酵实验和单细菌培养实验表明，吲哚-3-乳酸可降低肠道中多种 ARGs 的丰度和风险，并抑制 ARG 宿主大肠杆菌的生长。在人们高度关注肠道微生物代谢和抗生素抗性的背景下，本研究首次聚焦于蛋鸡肠道微生物代谢与抗生素抗性之间的关系。这些发现对健康养殖和抗生素抗性控制具有重要意义。
