Laboratory for Research on Advanced Processes for Water Treatment, Unidad Académica Juriquilla, Instituto de Ingeniería, Universidad Nacional Autónoma de México, Blvd. Juriquilla 3001, Querétaro 76230, Mexico.
Laboratory for Research on Advanced Processes for Water Treatment, Unidad Académica Juriquilla, Instituto de Ingeniería, Universidad Nacional Autónoma de México, Blvd. Juriquilla 3001, Querétaro 76230, Mexico.
Bioresour Technol. 2024 Sep;408:131224. doi: 10.1016/j.biortech.2024.131224. Epub 2024 Aug 5.
Lactic acid has been applied as a precursor for hydrogen (H) production from substrates rich in lactic acid bacteria (LAB), focusing on microbial interactions between producing and consuming LAB tested with model substrates. Therefore, this study evaluated the effect of single and combined lactic acid-consuming bacteria on mesophilic H production in batch tests from lactic acid from fermented food waste (FW). Megasphaera elsdenii, Clostridium beijerinckii, and Clostridium butyricum were inoculated at different ratios (v/v). Additionally, thermal pretreated sludge (TPS) was added to the strain mixtures. The highest production was obtained with M. elsdenii, C. beijerinckii, and C. butyricum (17:66:17 ratio), obtaining 1629.0 mL/L. The optimal mixture (68:32:0 of M. elsdenii and C. beijerinckii) enriched with TPS reached 1739.3 ± 98.6 mL H/L, consuming 98 % of lactic acid added. M. elsdenii and Clostridium strains enhance H production from lactic acid as they persist in a microbial community initially dominated by LAB.
乳酸已被用作富含乳酸菌（LAB）底物的氢气（H）生产的前体，重点是在模型底物上测试产酸和消耗 LAB 之间的微生物相互作用。因此，本研究评估了单种和混合乳酸消耗菌对分批发酵食品废物（FW）乳酸产生中温 H 的影响。接种不同比例（v/v）的巨大球型菌（Megasphaera elsdenii）、贝氏梭菌（Clostridium beijerinckii）和丁酸梭菌（Clostridium butyricum）。此外，还向菌株混合物中添加了热预处理污泥（TPS）。M. elsdenii、C. beijerinckii 和 C. butyricum（17:66:17 比例）的产量最高，达到 1629.0 mL/L。用 TPS 富集的最佳混合物（M. elsdenii 和 C. beijerinckii 的 68:32:0）达到 1739.3 ± 98.6 mL H/L，消耗添加的乳酸的 98%。M. elsdenii 和梭菌菌株通过在最初由 LAB 主导的微生物群落中持续存在来增强乳酸产生 H。
