Department of Chemistry and Life Science, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan.
FEMS Microbiol Lett. 2024 Jan 9;371. doi: 10.1093/femsle/fnae064.
The first steps in chitin degradation in marine bacteria involve chitinase, which produces N,N'-diacetylchitobiose (GlcNAc)2 from chitin. Moreover, in Vibrio bacteria, chitinase activity is enhanced by heterodisaccharide β-N-acetyl-d-glucosaminyl-(1,4)-d-glucosamine (GlcNAc-GlcN) produced from (GlcNAc)2 by chitin oligosaccharide deacetylase (COD). However, the role of COD in other marine bacteria, such as Shewanella, remains unexplored. This study investigates GlcNAc-GlcN's impact on chitinase gene expression and enzyme production in S. baltica ATCC BAA-1091, drawing parallels with Vibrio parahaemolyticus RIMD2210633. Using real-time quantitative PCR, the study assesses the upregulation of chitinase gene expression in S. baltica in response to GlcNAc-GlcN, informed by COD's known ability to produce GlcNAc-GlcN from (GlcNAc)2. In Vibrio, GlcNAc-GlcN considerably upregulates chitinase gene expression. This study posits a similar regulatory mechanism in S. baltica, with preliminary investigations indicating COD's capacity to produce GlcNAc-GlcN. This study highlights the importance of exploring GlcNAc-GlcN's regulatory role in chitin metabolism across diverse marine bacteria. The potential induction of chitinase production in S. baltica suggests broader ecological implications. Further research is crucial for a comprehensive understanding of chitin utilization and regulatory pathways in marine bacterial genera.
在海洋细菌中，几丁质降解的第一步涉及几丁质酶，它将几丁质分解为 N,N'-二乙酰壳二糖（GlcNAc）2。此外，在弧菌中，几丁质酶活性通过几丁质寡糖脱乙酰酶（COD）从（GlcNAc）2产生的杂二糖β-N-乙酰-d-葡萄糖胺基-（1,4）-d-葡萄糖胺（GlcNAc-GlcN）增强。然而，COD 在其他海洋细菌（如希瓦氏菌）中的作用仍未被探索。本研究调查了 GlcNAc-GlcN 对海洋细菌希瓦氏菌 ATCC BAA-1091 中几丁质酶基因表达和酶产生的影响，与副溶血弧菌 RIMD2210633 进行了比较。使用实时定量 PCR，本研究评估了 GlcNAc-GlcN 对 S. baltica 中几丁质酶基因表达的上调作用，这是基于 COD 已知能够从（GlcNAc）2 产生 GlcNAc-GlcN。在弧菌中，GlcNAc-GlcN 极大地上调了几丁质酶基因的表达。本研究假设在 S. baltica 中存在类似的调控机制，初步研究表明 COD 能够产生 GlcNAc-GlcN。本研究强调了探索 GlcNAc-GlcN 在不同海洋细菌中几丁质代谢中的调控作用的重要性。S. baltica 中几丁质酶产生的潜在诱导作用表明了更广泛的生态意义。进一步的研究对于全面了解海洋细菌属中几丁质的利用和调控途径至关重要。
