Department of Chemistry and Life Science, College of Bioresource Sciences, Nihon University, Kanagawa, Japan
Department of Chemistry and Life Science, College of Bioresource Sciences, Nihon University, Kanagawa, Japan.
J Bacteriol. 2019 Sep 20;201(20). doi: 10.1128/JB.00270-19. Print 2019 Oct 15.
RIMD2210633 secretes both chitinase and chitin oligosaccharide deacetylase and produces β--acetyl-d-glucosaminyl-(1,4)-d-glucosamine (GlcNAc-GlcN) from chitin. Previously, we reported that GlcNAc-GlcN induces chitinase production by several strains of harboring chitin oligosaccharide deacetylase genes (T. Hirano, K. Kadokura, T. Ikegami, Y. Shigeta, et al., Glycobiology 19:1046-1053, 2009). The metabolism of chitin by was speculated on the basis of the findings of previous studies, and the role of chitin oligosaccharide produced from chitin has been well studied. However, the role of GlcNAc-GlcN in the chitin degradation system, with the exception of the above-mentioned function as an inducer of chitinase production, remains unclear. ,'-Diacetylchitobiose, a homodisaccharide produced from chitin, is known to induce the expression of genes encoding several proteins involved in chitin metabolism in strains (K. L. Meibom, X. B. Li, A. Nielsen, C. Wu, et al., Proc Natl Acad Sci U S A 101:2524-2529, 2004). We therefore hypothesized that GlcNAc-GlcN also affects the expression of enzymes involved in chitin metabolism in the same manner. In this study, we examined the induction of protein expression by several sugars released from chitin using peptide mass fingerprinting and confirmed the expression of genes encoding enzymes involved in chitin metabolism using real-time quantitative PCR analysis. We then confirmed that GlcNAc-GlcN induces the expression of genes encoding many soluble enzymes involved in chitin degradation in Here, we demonstrate that GlcNAc-GlcN enhances the chitin-metabolizing ability of We demonstrate that β--acetyl-d-glucosaminyl-(1,4)-d-glucosamine (GlcNAc-GlcN) enhances the chitin-metabolizing ability of Members of the genus are chitin-degrading bacteria, and some species of this genus are associated with diseases affecting fish and animals, including humans (F. L. Thompson, T. Iida, and J. Swings, Microbiol Mol Biol Rev 68:403-431, 2004; M. Y. Ina-Salwany, N. Al-Saari, A. Mohamad, F.-A. Mursidi, et al., J Aquat Anim Health 31:3-22, 2019). Studies on are considered important, as they may facilitate the development of solutions related to health, food, and aquaculture problems attributed to this genus. This report enhances the current understanding of chitin degradation by bacteria.
RIMD2210633 分泌几丁质酶和几丁寡糖脱乙酰酶，并将几丁质转化为β--乙酰-d-氨基葡萄糖基-(1,4)-d-葡萄糖胺（GlcNAc-GlcN）。此前，我们报道了 GlcNAc-GlcN 可诱导携带几丁寡糖脱乙酰酶基因的[菌株产生几丁质酶（T. Hirano、K. Kadokura、T. Ikegami、Y. Shigeta 等人，Glycobiology 19:1046-1053, 2009）。根据先前研究的结果推测了[的几丁质代谢情况，并且已经很好地研究了几丁寡糖在几丁质代谢中的作用。然而，GlcNAc-GlcN 在[几丁质降解系统中的作用除了作为几丁质酶产生的诱导剂之外，仍然不清楚。几丁质二糖，一种由几丁质产生的同源二糖，已知可诱导[菌株中参与几丁质代谢的几种蛋白质基因的表达（K. L. Meibom、X. B. Li、A. Nielsen、C. Wu 等人，Proc Natl Acad Sci U S A 101:2524-2529, 2004）。因此，我们假设 GlcNAc-GlcN 也以相同的方式影响参与几丁质代谢的酶的表达。在这项研究中，我们使用肽质量指纹图谱检查了几种从几丁质释放的糖对蛋白质表达的诱导作用，并使用实时定量 PCR 分析证实了参与几丁质代谢的酶基因的表达。然后，我们证实 GlcNAc-GlcN 诱导[中许多参与几丁质降解的可溶性酶基因的表达。在这里，我们证明了 GlcNAc-GlcN 增强了[几丁质代谢能力。我们证明了β--乙酰-d-氨基葡萄糖基-(1,4)-d-葡萄糖胺（GlcNAc-GlcN）增强了[几丁质代谢能力。属的成员是几丁质降解细菌，该属的一些物种与影响鱼类和动物（包括人类）的疾病有关（F. L. Thompson、T. Iida 和 J. Swings， Microbiol Mol Biol Rev 68:403-431, 2004；M. Y. Ina-Salwany、N. Al-Saari、A. Mohamad、F.-A. Mursidi 等人，J Aquat Anim Health 31:3-22, 2019）。对[的研究被认为很重要，因为它们可能有助于解决与该属有关的健康、食品和水产养殖问题。本报告增强了对[细菌几丁质降解的现有理解。
