Unilever Research and Development, Trumbull, Connecticut, USA.
Unilever Research and Development, Colworth Science Park, Bedford, UK.
Int J Cosmet Sci. 2024 Aug;46(4):516-525. doi: 10.1111/ics.12962.
Atopic dermatitis (AD) is characterized by compositional and structural changes to the skin at lesional sites. Alteration to the levels and organization of both protein and lipid components are associated with disease status and lead to impaired barrier and hydration. Corneodesmosin (CDSN) and the arrangement and length of the intercellular lipid lamellae (ICLL) are altered in disrupted skin states. The aim of this research was to profile the distribution of CDSN and the ICLL in the stratum corneum (SC) at lesional and non-lesional sites in AD-prone skin and to investigate the impact of an eczema calming lotion containing petroleum jelly, fatty acids, and colloidal oatmeal.
An IRB-approved study was conducted with participants with active AD. From a small subset of participants, tape strips were collected from lesional and non-lesional sites on the arm, prior to and after twice daily application, over 4 weeks of an eczema calming lotion containing petroleum jelly, fatty acids, and colloidal oatmeal. Fluorescent antibody staining was used to investigate the distribution of CDSN. Transmission electron microscopy (TEM) was used to characterize the ICLL.
The distribution/coverage of CDSN was similar between lesional and non-lesional sites at baseline; application of the lotion resulted in a more defined honeycomb/peripheral distribution. Normalized ICLL (nICLL) was lower in baseline samples from lesional sites relative to non-lesional sites. Application of the lotion increased this parameter by the end of the study at all sites.
The eczema calming lotion containing petroleum jelly, fatty acids and colloidal oatmeal provided changes in corneodesmosomal proteins distribution and ICLL, consistent with improvements in corneocyte maturation and improved barrier function in the skin of individuals with atopic dermatitis.
特应性皮炎（AD）的特征是病变部位的皮肤存在组成和结构的变化。蛋白质和脂质成分的水平和组织的改变与疾病状态相关，并导致屏障和水合作用受损。桥粒芯糖蛋白（CDSN）和细胞间脂质层（ICLL）的排列和长度在皮肤紊乱状态下发生改变。本研究旨在描绘特应性皮炎倾向皮肤的病变和非病变部位的角层中 CDSN 和 ICLL 的分布，并研究含有凡士林、脂肪酸和胶体燕麦片的湿疹舒缓乳液的影响。
对患有活动性 AD 的参与者进行了一项经机构审查委员会批准的研究。从小部分参与者中，在手臂的病变和非病变部位采集胶带条，在含有凡士林、脂肪酸和胶体燕麦片的湿疹舒缓乳液每日两次应用 4 周之前和之后。荧光抗体染色用于研究 CDSN 的分布。透射电子显微镜（TEM）用于表征 ICLL。
在基线时，病变和非病变部位的 CDSN 分布/覆盖率相似；涂抹乳液后，出现更明确的蜂窝状/周边分布。与非病变部位相比，病变部位的基线样本的归一化 ICLL（nICLL）较低。在研究结束时，所有部位应用乳液后均增加了该参数。
含有凡士林、脂肪酸和胶体燕麦片的湿疹舒缓乳液可改变桥粒芯糖蛋白分布和 ICLL，这与特应性皮炎患者皮肤中角质形成细胞成熟和改善屏障功能的改善一致。
