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紫花苜蓿基因型对铝毒胁迫耐受性的评价与筛选

Evaluation and selection of alfalfa genotypes for tolerance to aluminium toxic stress.

作者信息

Liatukienė Aurelija, Skuodienė Regina, Norkevičienė Eglė, Tamm Sirje, Pechter Priit, Petrauskas Giedrius

机构信息

Lithuanian Research Centre for Agriculture and Forestry, Institute of Agriculture, Instituto al. 1, Kėdainiai dist., Lithuania.

Lithuanian Research Centre for Agriculture and Forestry, Vėžaičiai Branch, Klaipėda dist., Lithuania.

出版信息

Front Plant Sci. 2024 Jul 24;15:1437993. doi: 10.3389/fpls.2024.1437993. eCollection 2024.

DOI:10.3389/fpls.2024.1437993
PMID:39114475
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11305175/
Abstract

Alfalfa is one of the most important and the most cultivated crop due to its high nutritive quality and yield, but adaptation of alfalfa genotypes differ in terms of mobile aluminium stress in the soil. The aim of this study was to evaluate the tolerance to mobile Al concentrations in the laboratory and in the naturally acidic soil and select the promising genotypes based on agro-biological traits. In 2019, a laboratory experiment was conducted at the Institute of Agriculture of LAMMC. The experiment in the acidic soil with different mobile Al concentrations was conducted at the Vėžaičiai Branch of LAMMC. In 2020, the crops of alfalfa genotypes (11 cultivars and 3 populations) were established on . The agro-biological traits were assessed during the 2021-2022 season. The tolerance index of hypocotyls and roots was evaluated using the filter-based screening method at different AlCl (0.0-64 mM) concentrations. The study results of the filter-based screening method showed that the genotype Žydrūnė, Malvina, Jõgeva 118, Skriveru, and 3130 were the most tolerant ones and the hypocotyl tolerance index of these genotypes was higher compared to medium tolerant genotypes Birutė, PGR12489, Europe and AJ2024 at 8, 16, 32 and 64 mM AlCl concentrations. The hypocotyl and root tolerance index of medium tolerant genotypes was higher compared to a sensitive genotype PGR10249 at 8 and 16 mM AlCl. The study of cluster analysis with mobile Al 0.0-65.0 mg kg showed that the genotypes Žydrūnė, Europe, AJ2024 and 3130 were the best in terms of wintering and spring regrowth, the cultivar Malvina had the best value of wintering, height before flowering and stem number, the cultivar Birutė had the best value of spring regrowth, height before flowering and seed yield, and the cultivar Skriveru had the best value of spring regrowth, height before flowering, stem number and seed yield.

摘要

紫花苜蓿因其高营养价值和产量,是最重要且种植最广泛的作物之一,但不同紫花苜蓿基因型对土壤中活性铝胁迫的适应性存在差异。本研究的目的是在实验室和天然酸性土壤中评估对活性铝浓度的耐受性,并根据农业生物学性状筛选出有潜力的基因型。2019年,在拉脱维亚农业大学作物科学研究所进行了一项实验室试验。在拉脱维亚农业大学韦扎伊čiai分校对不同活性铝浓度的酸性土壤进行了试验。2020年,在……上种植了紫花苜蓿基因型(11个品种和3个群体)的作物。在2021 - 2022季评估了农业生物学性状。在不同AlCl₃(0.0 - 64 mM)浓度下,使用基于滤纸的筛选方法评估下胚轴和根的耐受性指数。基于滤纸筛选方法的研究结果表明,Žydrūnė、Malvina、Jõgeva 118、Skriveru和3130基因型耐受性最强,在8、16、32和64 mM AlCl₃浓度下,这些基因型的下胚轴耐受性指数高于中等耐受性基因型Birutė、PGR12489、Europe和AJ2024。在8和16 mM AlCl₃浓度下,中等耐受性基因型的下胚轴和根耐受性指数高于敏感基因型PGR10249。对活性铝含量为0.0 - 65.0 mg/kg的聚类分析研究表明,就越冬和春季再生而言,Žydrūnė、Europe、AJ2024和3130基因型表现最佳;品种Malvina在越冬、开花前高度和茎数方面表现最佳;品种Birutė在春季再生、开花前高度和种子产量方面表现最佳;品种Skriveru在春季再生、开花前高度、茎数和种子产量方面表现最佳。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3426/11305175/1fd70ef4d4b5/fpls-15-1437993-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3426/11305175/0a4b3d6daed0/fpls-15-1437993-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3426/11305175/8fb33298b39d/fpls-15-1437993-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3426/11305175/e1a4c0dde176/fpls-15-1437993-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3426/11305175/74aa892a1237/fpls-15-1437993-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3426/11305175/c5b42751fa57/fpls-15-1437993-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3426/11305175/7327f02d8ff6/fpls-15-1437993-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3426/11305175/e12f0bc9f66c/fpls-15-1437993-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3426/11305175/6625d164268b/fpls-15-1437993-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3426/11305175/1fd70ef4d4b5/fpls-15-1437993-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3426/11305175/0a4b3d6daed0/fpls-15-1437993-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3426/11305175/8fb33298b39d/fpls-15-1437993-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3426/11305175/e1a4c0dde176/fpls-15-1437993-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3426/11305175/74aa892a1237/fpls-15-1437993-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3426/11305175/c5b42751fa57/fpls-15-1437993-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3426/11305175/7327f02d8ff6/fpls-15-1437993-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3426/11305175/e12f0bc9f66c/fpls-15-1437993-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3426/11305175/6625d164268b/fpls-15-1437993-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3426/11305175/1fd70ef4d4b5/fpls-15-1437993-g009.jpg

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