Fan Chanjuan, Li Zhen, Zhai Lili, Wang Hui, Zhao Xiaolin, Xie Dongling, Cai Yong, Huang Kun, Bai Qixuan, Ding Haiou, Cheng Jianping
Department of Gastroenterology and Oncology, Civil Aviation General Hospital Beijing 100123, China.
Int J Clin Exp Pathol. 2024 Jul 15;17(7):219-226. doi: 10.62347/CLCL4783. eCollection 2024.
() is a globally prevalent bacterium that increases the risk of developing various gastrointestinal diseases, including gastric adenocarcinoma. This study aimed to evaluate the performances of real-time PCR assay in detecting infection, as well as clarithromycin and levofloxacin resistance, in both stool and gastric biopsy specimens.
Stool and gastric biopsy specimens were collected from patients within one to three days post-hospitalization. All patients were analyzed for infection and resistance to clarithromycin and levofloxacin using a real-time PCR based molecular assay.
169 patients (83 males) with a mean age of 43.6±13.1 years were included in the study. The prevalence of was 89.9% (152/169) in stool and 90.5% (153/169) in gastric biopsy samples. The molecular diagnostics employed in this study exhibited a sensitivity of 99.3% and a specificity of 100%, resulting in a diagnostic accuracy rate of 99.6%. Resistance to clarithromycin was 36.1% (61/169) in stool and 44.4% (75/169) in gastric biopsy samples. The molecular tests for clarithromycin resistance demonstrated a sensitivity of 96.8% and a specificity of 86.8%, with an overall diagnostic accuracy of 90.5%. Furthermore, resistance to levofloxacin was 22.5% (38/169) and 26.6% (45/169) in stool and gastric biopsy samples, respectively. The molecular test demonstrated a sensitivity of 80.9% and a specificity of 94.3%, resulting in a diagnostic accuracy of 90.5%.
The implementation of real-time PCR-based screening for infection and resistance to clarithromycin and levofloxacin in the stool may enhance the success rate of eradication therapy.
（某种细菌名称未给出）是一种全球流行的细菌，会增加患各种胃肠道疾病（包括胃腺癌）的风险。本研究旨在评估实时荧光定量聚合酶链反应（PCR）检测法在检测粪便和胃活检标本中该细菌感染以及克拉霉素和左氧氟沙星耐药性方面的性能。
在患者住院后1至3天内收集粪便和胃活检标本。使用基于实时荧光定量PCR的分子检测法对所有患者进行该细菌感染以及对克拉霉素和左氧氟沙星耐药性的分析。
本研究纳入了169例患者（83例男性），平均年龄为43.6±13.1岁。粪便中该细菌的患病率为89.9%（152/169），胃活检样本中为90.5%（153/169）。本研究采用的分子诊断方法灵敏度为99.3%，特异性为100%，诊断准确率为99.6%。粪便中对克拉霉素的耐药率为36.1%（61/169），胃活检样本中为44.4%（75/169）。克拉霉素耐药性的分子检测灵敏度为96.8%，特异性为86.8%，总体诊断准确率为90.5%。此外，粪便和胃活检样本中对左氧氟沙星的耐药率分别为22.5%（38/169）和26.6%（45/169）。分子检测灵敏度为80.9%，特异性为94.3%，诊断准确率为90.5%。
在粪便中实施基于实时荧光定量PCR的该细菌感染以及克拉霉素和左氧氟沙星耐药性筛查，可能会提高根除治疗的成功率。
