Biochemistry, Cell and Systems Biology, Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Liverpool, UK.
Biochemistry Section, Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, USA.
Methods Mol Biol. 2024;2845:141-150. doi: 10.1007/978-1-0716-4067-8_11.
We outline our approach for studying the selective autophagy of peroxisomes (pexophagy), using fluorescence microscopy in tissue cell culture models. Ratiometric reporters, which specifically localize to peroxisomes, allow a quantitative assessment of pexophagy in fixed and live cells, as well as whole organisms. We discuss chemical and physiological inducers of pexophagy and any overlap with the induction of mitophagy.
我们概述了使用组织细胞培养模型中的荧光显微镜研究过氧化物酶体（pexophagy）选择性自噬的方法。特异性定位于过氧化物酶体的比率报告器允许对固定和活细胞以及整个生物体中的pexophagy 进行定量评估。我们讨论了pexophagy 的化学和生理诱导物以及与线粒体自噬诱导的重叠。
