Interdisciplinary Laboratory of Clinical Analysis, Interlab-UMU, Regional Campus of International Excellence Campus Mare Nostrum, University of Murcia, 30100 Murcia, Spain.
Interdisciplinary Laboratory of Clinical Analysis, Interlab-UMU, Regional Campus of International Excellence Campus Mare Nostrum, University of Murcia, 30100 Murcia, Spain; Department of Animal Production, Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, Campus de Espinardo s/n, 30100.
Domest Anim Endocrinol. 2024 Oct;89:106875. doi: 10.1016/j.domaniend.2024.106875. Epub 2024 Jul 16.
The activity of the enzyme 11β-hydroxysteroid dehydrogenase type II (11β-HSD type II), which can be estimated by the combined measurement of cortisol and cortisone, is gaining importance as a marker for the assessment of stress in pigs. The aim of this study was to investigate the activity of this enzyme and the salivary concentrations of cortisol and cortisone in pigs during pregnancy, farrowing and lactation and to compare it with other stress-related biomarkers such as Chromogranin A (CgA), S100A12 and alpha-amylase. Salivary cortisone concentrations and 11β-HSD type II activity decreased after farrowing, while cortisol concentrations increased. Enzyme activity did not show significant correlations with any of the other stress-related biomarkers measured in this study. Overall, the results of this report indicate a different regulation of 11β-HSD type II activity and of cortisol and cortisone during pregnancy and lactation, which should be considered when evaluating these analytes in saliva during these periods.
11β-羟类固醇脱氢酶 II 型（11β-HSD 类型 II）的活性可以通过皮质醇和可的松的联合测量来评估，它作为评估猪应激的标志物越来越受到重视。本研究旨在调查妊娠、分娩和哺乳期猪的这种酶的活性以及唾液中的皮质醇和可的松浓度，并将其与其他应激相关生物标志物如嗜铬粒蛋白 A（CgA）、S100A12 和α-淀粉酶进行比较。分娩后，唾液中可的松浓度和 11β-HSD 类型 II 活性降低，而皮质醇浓度升高。酶活性与本研究中测量的其他应激相关生物标志物均无显著相关性。总的来说，本报告的结果表明，在妊娠和哺乳期，11β-HSD 类型 II 活性以及皮质醇和可的松的调节存在差异，在这些时期评估唾液中的这些分析物时应考虑到这一点。
