López-Arjona M, Tecles F, Mateo S V, Contreras-Aguilar M D, Martínez-Miró S, Cerón J J, Martínez-Subiela S
Interdisciplinary Laboratory of Clinical Analysis of the University of Murcia (Interlab-UMU), Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, Campus de Espinardo s/n, 30100 Espinardo, Murcia, Spain.
Departament of Animal Production, Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, Campus de Espinardo s/n, 30100 Espinardo, Murcia, Spain.
Vet J. 2020 May-Jun;259-260:105458. doi: 10.1016/j.tvjl.2020.105458. Epub 2020 May 19.
Two sensitive assays based on AlphaLISA technology were developed and validated for the measurement of cortisol and cortisone in hair of pigs, that also enabled estimation of 11β-hydroxysteroid dehydrogenase type 2 activity. These assays were applied to hair samples from sows (n = 32) collected at 5 days before, and at 23 and 59 after farrowing, in reproductive cycles in two different periods: spring-summer (n = 16) and winter-spring (n = 16). The assays were precise (imprecision <12%) and accurate (recovery range, 80-115%) for cortisol and cortisone determination. Hair cortisone concentrations and the cortisone/cortisol ratio (an estimate of 11β-hydroxysteroid dehydrogenase isoenzyme type 2 activity) increased after farrowing more than cortisol, being these changes of higher magnitude during periods of higher atmospheric temperature. The measurement of hair cortisone concentrations and estimations of the activity of the 11β-hydroxysteroid dehydrogenase isoenzyme type 2, measured by the assays developed in this study, are complementary biomarkers to hair cortisol, and can increase at periods associated with stress, such as farrowing and lactation, especially at high atmospheric temperatures. .
开发并验证了两种基于AlphaLISA技术的灵敏检测方法，用于测定猪毛中的皮质醇和可的松，这两种方法还能估算2型11β-羟基类固醇脱氢酶的活性。将这些检测方法应用于两个不同时期（春夏季，n = 16；冬春季，n = 16）繁殖周期中母猪（n = 32）的毛发样本，这些样本分别在分娩前5天、分娩后23天和59天采集。对于皮质醇和可的松的测定，这些检测方法具有精密度（不精密度<12%）和准确度（回收率范围为80-115%）。分娩后，毛发中可的松浓度和可的松/皮质醇比值（对2型11β-羟基类固醇脱氢酶同工酶活性的一种估算）的增加幅度大于皮质醇，在气温较高时期，这些变化幅度更大。通过本研究开发的检测方法测定的毛发可的松浓度以及对2型11β-羟基类固醇脱氢酶同工酶活性的估算，是毛发皮质醇的补充生物标志物，并且在与应激相关的时期（如分娩和哺乳）会增加，尤其是在高气温时。
