School of Allied Sciences, Dev Bhoomi Uttarakhand University, Dehradun 248007, India.
Department of Pathology and Immunology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA.
Comput Biol Chem. 2024 Oct;112:108161. doi: 10.1016/j.compbiolchem.2024.108161. Epub 2024 Jul 25.
Deinococcus species, noted for their exceptional resistance to DNA-damaging environmental stresses, have piqued scientists' interest for decades. This study dives into the complex mechanisms underpinning radiation resistance in the Deinococcus genus. We have examined the genomes of 82 Deinococcus species and classified radiation-resistance proteins manually into five unique curated categories: DNA repair, oxidative stress defense, Ddr and Ppr proteins, regulatory proteins, and miscellaneous resistance components. This classification reveals important information about the various molecular mechanisms used by these extremophiles which have been less explored so far. We also investigated the presence or lack of these proteins in the context of phylogenetic relationships, core, and pan-genomes, which offered light on the evolutionary dynamics of radiation resistance. This comprehensive study provides a deeper understanding of the genetic underpinnings of radiation resistance in the Deinococcus genus, with potential implications for understanding similar mechanisms in other organisms using an interactomics approach. Finally, this study reveals the complexities of radiation resistance mechanisms, providing a comprehensive understanding of the genetic components that allow Deinococcus species to flourish under harsh environments. The findings add to our understanding of the larger spectrum of stress adaption techniques in bacteria and may have applications in sectors ranging from biotechnology to environmental research.
多年来，耐辐射球菌因其对 DNA 损伤环境压力的非凡抗性而引起了科学家的兴趣。本研究深入探讨了耐辐射球菌属辐射抗性的复杂机制。我们研究了 82 种耐辐射球菌的基因组，并手动将辐射抗性蛋白分类为五个独特的已分类类别：DNA 修复、氧化应激防御、Ddr 和 Ppr 蛋白、调节蛋白和杂类抗性成分。这种分类揭示了有关这些极端微生物迄今为止探索较少的各种分子机制的重要信息。我们还研究了这些蛋白质在系统发育关系、核心基因组和泛基因组中的存在或缺失情况，这为辐射抗性的进化动态提供了一些线索。这项全面的研究提供了对耐辐射球菌属辐射抗性遗传基础的更深入了解，这可能有助于通过相互作用组学方法了解其他生物体中类似的机制。最后，这项研究揭示了辐射抗性机制的复杂性，全面了解了使耐辐射球菌属在恶劣环境中繁衍生息的遗传成分。这些发现增加了我们对细菌中更大范围的应激适应技术的理解，并可能在生物技术到环境研究等领域有应用。
