Wang Wuzhou, Du Zhenfang, Yang Fei, He Shuya
Department of Biochemistry and Molecular Biology, School of Basic Medicine, Hengyang Medical School, University of South China, Hengyang, 421001, Hunan, China.
School of Pharmaceutical Technology, Hunan Polytechnic of Environment and Biology, Hengyang, 421005, Hunan, China.
Antonie Van Leeuwenhoek. 2025 Jul 18;118(9):116. doi: 10.1007/s10482-025-02125-5.
Two-component system (TCS) plays a crucial role in translating stimulus signals into specific adaptive responses and aids prokaryotic organisms in withstanding diverse stresses. Deinococcus radiodurans is renowned for its exceptional radioresistance and has become a valuable model for studying DNA repair, bioremediation and planetary protection space missions. TCSs are well-developed by this bacterium, thus the role in its radioresistance can be envisaged. DR2416 was predicted to be a histidine kinase, and DR2415, a response regulator. Our study revealed that deleting dr2416 or (and) dr2415 reduced D. radiodurans' resistance to various DNA damage agents, including ionizing radiation, hydrogen peroxide, and mitomycin C. The DR2416 protein was situated on the membrane of D. radiodurans. Western blotting analysis revealed that DR2415 was significantly induced by ionizing radiation. The Phos-tag SDS-PAGE assay illustrated that DR2416 could undergo self-phosphorylation at His259 in vitro and subsequently transfer the phosphoryl group to the Asp52 residue of DR2415. The qRT-PCR method showed that the DR2416/DR2415 TCS regulated genes related to DNA double-strand break repair and reactive oxygen species (ROS) removal, including recN (dr1477), sbcC (dr1922), and katE (dra0259). In conclusion, our findings suggested that the DR2416/DR2415 TCS might provide radioresistance to D. radiodurans through DNA damage repair and ROS scavenging mechanisms.
双组分系统（TCS）在将刺激信号转化为特定的适应性反应中起着关键作用，并帮助原核生物抵御各种压力。耐辐射球菌以其卓越的抗辐射能力而闻名，已成为研究DNA修复、生物修复和行星保护太空任务的宝贵模型。这种细菌的双组分系统发育良好，因此可以设想其在抗辐射中的作用。DR2416被预测为一种组氨酸激酶，而DR2415是一种反应调节因子。我们的研究表明，删除dr2416或（和）dr2415会降低耐辐射球菌对各种DNA损伤剂的抗性，包括电离辐射、过氧化氢和丝裂霉素C。DR2416蛋白位于耐辐射球菌的细胞膜上。蛋白质免疫印迹分析表明，DR2415受到电离辐射的显著诱导。Phos-tag SDS-PAGE分析表明，DR2416在体外可在His259处进行自身磷酸化，随后将磷酸基团转移至DR2415的Asp52残基上。qRT-PCR方法表明，DR2416/DR2415双组分系统调节与DNA双链断裂修复和活性氧（ROS）清除相关的基因，包括recN（dr1477）、sbcC（dr1922）和katE（dra0259）。总之，我们的研究结果表明，DR2416/DR2415双组分系统可能通过DNA损伤修复和ROS清除机制为耐辐射球菌提供抗辐射能力。
