Godoy Bianca L V, Barroso Marlon do Valle, Dantas Karine, Rodrigues Vitória G T, Ferreira Taís P, Atuí Caroline, Valle Ana C, Fuga Bruna, Lincopan Nilton, Nogueira Mara C L, Casella Tiago
Faculdade de Medicina de São José do Rio Preto, Centro de Investigação de Microrganismos, São José do Rio Preto, SP, Brazil.
Universidade de São Paulo, Instituto de Ciências Biomédicas II, São Paulo, SP, Brazil; One Health Brazilian Resistance Project (OneBR), Brazil.
J Glob Antimicrob Resist. 2024 Dec;39:3-5. doi: 10.1016/j.jgar.2024.07.015. Epub 2024 Aug 6.
Klebsiella spp. are leading causes of nosocomial infections. Their ability to harbour antimicrobial resistance genes makes them an important public health threat. This study aimed to report the genomic background of carbapenemase-producing Klebsiella quasipneumoniae (HV55B) and Klebsiella michiganensis (HV55D) strains isolated from fresh vegetables destined for hospitalized inpatients.
Microbiological and molecular methods were used to isolate and identify the strains, which were submitted to the antimicrobial susceptibility test and pH tolerance assays. Whole genome sequencing was performed on MiSeq and NextSeq platforms, and online available tools were applied to bioinformatic analysis of clinically relevant information.
Both isolates were considered multidrug-resistant and tolerated pH ≥ 4 for 24 h. HV55B belonged to sequence type (ST) ST668, and presented a broad resistome and plasmids from four incompatibility groups. HV55D belonged to ST40. Both strains HV55B and HV55D were genetically close to isolates responsible for human infections around the world, which stands for the plausibility of such bacteria to cause disease in patients of the studied institution.
Our results confirm the presence of carbapenemase-producing Klebsiella spp. in fresh foodstuffs intended for hospitalized inpatients' consumption. The genomes characterized here also provide clinically and genomically relevant information to forthcoming epidemiological surveillance studies.
克雷伯菌属是医院感染的主要致病菌。其携带抗菌药物耐药基因的能力使其成为重要的公共卫生威胁。本研究旨在报告从供住院患者食用的新鲜蔬菜中分离出的产碳青霉烯酶肺炎克雷伯菌（HV55B）和密歇根克雷伯菌（HV55D）菌株的基因组背景。
采用微生物学和分子方法分离并鉴定菌株，对其进行药敏试验和pH耐受性测定。在MiSeq和NextSeq平台上进行全基因组测序，并应用在线可用工具对临床相关信息进行生物信息学分析。
两种分离株均被认为具有多重耐药性，且在pH≥4的条件下可耐受24小时。HV55B属于序列型（ST）ST668，具有广泛的耐药组和来自四个不相容群的质粒。HV55D属于ST40。HV55B和HV55D菌株在基因上均与世界各地引起人类感染的分离株相近，这表明此类细菌有可能在该研究机构的患者中引起疾病。
我们的数据证实了供住院患者食用的新鲜食品中存在产碳青霉烯酶的克雷伯菌属。此处表征的基因组也为即将开展的流行病学监测研究提供了临床和基因组相关信息。
