Ashton I K, Zapf J, Einschenk I, MacKenzie I Z
Acta Endocrinol (Copenh). 1985 Dec;110(4):558-63. doi: 10.1530/acta.0.1100558.
IGF-1 and IGF-2 were measured by specific radioimmunoassay after acid-ethanol extraction of plasma obtained by foetoscopy from 20 normal foetuses aged 15-23 weeks. IGF-1 and IGF-2 levels were 36 +/- 11 and 162 +/- 55 ng/ml, respectively. In comparison, levels in cord blood were 84 +/- 58 and 264 +/- 176 ng/ml, respectively, and in adult plasma were 410 +/- 106 and 818 +/- 272 ng/ml. Both IGF-1 and IGF-2 were in the normal foetal range in a further three foetuses with anencephaly and two foetuses with spina bifida. No sex difference was observed. IGF-1 was positively correlated with foetal body weight (P less than 0.001), placenta weight (P less than 0.02) and with body length measured crown-rump (P less than 0.01) or crown-heel (P less than 0.02). No correlation between IGF-2 and body weight, length, placenta weight or gestational age was found. Both IGF-1 and IGF-2 are present in the human foetal circulation earlier in gestation than has previously been demonstrated, the levels being low throughout this period of gestation in comparison with adult plasma.
通过对20例15至23周龄正常胎儿进行胎儿镜检查获取血浆，经酸乙醇提取后，采用特异性放射免疫分析法测定胰岛素样生长因子-1（IGF-1）和胰岛素样生长因子-2（IGF-2）。IGF-1和IGF-2水平分别为36±11和162±55纳克/毫升。相比之下，脐血中的水平分别为84±58和264±176纳克/毫升，成人血浆中的水平分别为410±106和818±272纳克/毫升。另外3例无脑儿胎儿和2例脊柱裂胎儿的IGF-1和IGF-2均处于正常胎儿范围内。未观察到性别差异。IGF-1与胎儿体重（P<0.001）、胎盘重量（P<0.02）以及顶臀长度（P<0.01）或顶跟长度（P<0.02）呈正相关。未发现IGF-2与体重、身长、胎盘重量或孕周之间存在相关性。与之前所证实的情况相比，IGF-1和IGF-2在人类胎儿循环中出现的时间更早，在整个孕期中其水平与成人血浆相比均较低。
