Mincy Callie, Revelt Luke, Carter Kathryn, Reed Donald, Joy Anita
Department of Growth, Development and Structure, Southern Illinois University School of Dental Medicine, Alton, IL USA.
Department of Biological Sciences, College of Arts and Sciences, Southern Illinois University Edwardsville, Edwardsville, IL USA.
J Maxillofac Oral Surg. 2024 Aug;23(4):896-908. doi: 10.1007/s12663-024-02250-0. Epub 2024 Jun 16.
The characterization of circulating tumor cells (CTC) and circulating tumor microemboli (CTM) has emerged as both a challenge to the standard view of metastasis, and as a valuable means for understanding genotypic and phenotypic variability shown even within the same cancer type. However, in the case of salivary gland neoplasms, limited data are available for the role that CTCs and CTMs play in metastasis and secondary tumor formation.ru.AQ1 In response to this, we propose that similarities between in vitro clusters of cultured salivary gland cancer cells may act as a surrogate model for in vivo CTCs and CTMs isolated from patients.
Using techniques in immunofluorescence, immunoblotting, and 2-dimensional migration, we isolated and characterized a group of cohort cells from a commercially available cell line (HTB-41). Results: Here, cells exhibited a hybrid phenotype with simultaneous expression of both epithelial and mesenchymal markers (E-cadherin, vimentin, and α-SMA). Cohort cells also exhibited increased migration in comparison to parental cells.
Data suggest that these isolated cell clusters may fucntion as a potential in vitro model of CTCs and CTMs.
循环肿瘤细胞（CTC）和循环肿瘤微栓子（CTM）的特征已成为对转移标准观点的挑战，同时也是理解即使在同一癌症类型中也存在的基因型和表型变异性的宝贵手段。然而，对于唾液腺肿瘤，关于CTC和CTM在转移和继发性肿瘤形成中所起作用的数据有限。针对这一点，我们提出，培养的唾液腺癌细胞体外簇之间的相似性可能作为从患者体内分离出的CTC和CTM的替代模型。
我们使用免疫荧光、免疫印迹和二维迁移技术，从一种市售细胞系（HTB - 41）中分离并鉴定了一组队列细胞。结果：在此，细胞表现出混合表型，同时表达上皮和间充质标志物（E - 钙黏蛋白、波形蛋白和α - 平滑肌肌动蛋白）。与亲代细胞相比，队列细胞的迁移能力也有所增强。
数据表明，这些分离的细胞簇可能作为CTC和CTM的潜在体外模型发挥作用。
