Keerthika R, Devi Anju, Kamboj Mala, Narwal Anjali
Department of Oral Pathology and Microbiology, Faculty of Dental Sciences, Trauma Centre, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh 221005 India.
Department of Oral Pathology and Microbiology, Post Graduate Institute of Dental Sciences (PGIDS), Pt. Bhagwat Dayal Sharma University of Health Sciences, Rohtak, Haryana 124001 India.
J Maxillofac Oral Surg. 2024 Aug;23(4):881-889. doi: 10.1007/s12663-024-02217-1. Epub 2024 Jun 21.
Cellular cannibalism (CC) is a prime metabolic event to determine the aggressive potential of oral squamous cell carcinoma (OSCC). However, the etiology and mechanism behind this degradation are still ambiguous. The aim of the study was to explore the etiopathogenetic mechanism behind CC, along with its association with degree of differentiation, angiogenic, phagocytic and antiapoptotic activity in OSCC.
Seventy-three tissue sections of various histological grades of OSCC were retrieved from departmental archives and scanned for cannibalistic cells. Immunohistochemical analysis using CD31, CD68, and BCL2 was performed. The data obtained were analyzed using Chi-square, Spearman's correlation test and multiple regression analysis ( < 0.05).
CCs were present significantly in various grades of OSCC ( < 0.00). Immunohistochemical analysis revealed a significant difference in CD68, BCL2 ( < 0.05 in both), and CD31 ( < 0.001) expression with CC. The internalized cells showed positivity for CD68 and negativity for BCL2. Regression analysis revealed that tumor grade, CD31 and BCL2 immunoreactivity were significant predictors of frequency of CC.
The association of CC with degree of differentiation, CD31, CD68, and BCL2 expression could predict the biological behavior of OSCC and might serve as a promising histopathological parameter in future.
细胞吞噬作用（CC）是决定口腔鳞状细胞癌（OSCC）侵袭潜能的主要代谢事件。然而，这种降解背后的病因和机制仍不明确。本研究的目的是探讨CC背后的病因发病机制，以及其与OSCC分化程度、血管生成、吞噬和抗凋亡活性的关系。
从科室档案中检索出73份不同组织学分级的OSCC组织切片，并对吞噬细胞进行扫描。采用CD31、CD68和BCL2进行免疫组织化学分析。对获得的数据进行卡方检验、Spearman相关检验和多元回归分析（<0.05）。
CC在不同分级的OSCC中均显著存在（<0.00）。免疫组织化学分析显示，CC组CD68、BCL2（两者均<0.05）和CD31（<0.001）表达存在显著差异。内化细胞CD68呈阳性，BCL2呈阴性。回归分析显示，肿瘤分级、CD31和BCL2免疫反应性是CC频率的重要预测因素。
CC与分化程度、CD31、CD68和BCL2表达的相关性可预测OSCC的生物学行为，未来可能成为一个有前景的组织病理学参数。
