Sarode Sachin C, Sarode Gargi S
Department of Oral Pathology and Microbiology, Dr. D. Y. Patil Dental College and Hospital, Dr. D.Y. Patil Vidyapeeth, Pimpri, Pune, India.
J Oral Pathol Med. 2014 Jul;43(6):459-63. doi: 10.1111/jop.12119. Epub 2013 Sep 20.
Cancer cannibalism is used to differentiate benign tumors from malignant, but recently the phenomenon has been demonstrated in giant cell tumor of tendon sheath (localized type). Microscopically and pathogenetically, this tumor is similar to central giant cell granuloma (CGCG) and peripheral giant cell granuloma (PGCG) of oral cavity. Hence, attempt has been made to study the cannibalistic giant cells (GCs) in CGCG and PGCG with their correlation with the biological behavior.
Surgically treated 16 CGCG and 23 PGCG cases with adequate clinical and radiographic documentation were selected. Quantification of cannibalistic GCs was performed using routine HE stain. Hundred GCs were examined in each section, and number of cannibalistic cells was expressed in percentage. Ten cases were randomly selected for further immunohistochemical analysis with CD68 and bcl-2.
Cannibalism was found in all the cases (100%). The frequency of occurrence of cannibalistic GCs ranged from 20% to 56% with a mean of 33.62 ± 8.9. CGCG showed significantly higher mean cannibalistic GC frequency (38.06 ± 10.15) than PGCG (30.04 ± 5.63). In aggressive CGCG, mean cannibalistic GC frequency was significantly higher (42.20 ± 10.4) than non-aggressive type (31.17 ± 6.014). Similarly, recurrent cases showed significantly higher mean cannibalistic cell frequency (43 ± 6.26) than non-recurrent cases (30.81 ± 6.66). Immunohistochemistry results showed histiocytic nature of GCs as well as mononuclear cells. The internalized cells did not expressed bcl-2, suggesting that the internalization induces apoptotic cell death.
Assessment of frequency of cannibalistic cells in CGCG and PCGC could help in predicting the biological behavior of the tumor.
癌症自噬现象被用于区分良性肿瘤和恶性肿瘤，但最近在腱鞘巨细胞瘤（局限性类型）中也发现了这一现象。在显微镜下和发病机制方面，这种肿瘤与口腔中央巨细胞肉芽肿（CGCG）和外周巨细胞肉芽肿（PGCG）相似。因此，人们试图研究CGCG和PGCG中的自噬性巨细胞（GCs）及其与生物学行为的相关性。
选取16例CGCG和23例PGCG病例，这些病例均接受了手术治疗，且有充分的临床和影像学记录。使用常规苏木精-伊红染色对自噬性GCs进行定量分析。在每个切片中检查100个GCs，自噬细胞的数量以百分比表示。随机选取10例病例进行CD68和bcl-2的进一步免疫组织化学分析。
所有病例（100%）均发现自噬现象。自噬性GCs的出现频率在20%至56%之间，平均为33.62±8.9。CGCG的自噬性GCs平均频率（38.06±10.15）显著高于PGCG（30.04±5.63）。在侵袭性CGCG中，自噬性GCs平均频率（42.20±10.4）显著高于非侵袭性类型（31.17±6.014）。同样，复发病例的自噬细胞平均频率（43±6.26）显著高于非复发病例（30.81±6.66）。免疫组织化学结果显示GCs以及单核细胞具有组织细胞性质。内化细胞不表达bcl-2，表明内化诱导凋亡性细胞死亡。
评估CGCG和PCGC中自噬细胞的频率有助于预测肿瘤的生物学行为。
