Hosseinzadeh M, Titidej A Z, Mokhlesi A, Yazdi Z, Azmoudeh F, Samani S, Saffar H
Student Research Committee, Qazvin University of Medical Sciences, Qazvin, Iran.
Department of Oral and Maxillofacial Pathology, Dental Caries Prevention Research Center, Qazvin University of Medical Sciences, Qazvin, Iran.
Arch Razi Inst. 2024 Dec 31;79(6):1345-1351. doi: 10.32592/ARI.2024.79.6.1345. eCollection 2024 Dec.
Tumor-associated tissue eosinophilia (TATE) has been associated with various tumors, including oral squamous cell carcinoma. However, the precise role of TATE in these contexts remains to be fully elucidated. The present study was undertaken to investigate the presence of tumor-associated tissue eosinophilia in oral squamous cell carcinomas (OSCCs) and its correlation with histopathologic grading. A total of 70 OSCC tissue samples were collected between 2016 and 2020 for examination. The samples comprised 60 previously diagnosed cases of OSCC, classified as well-differentiated squamous cell carcinoma (WDSCC), moderately differentiated squamous cell carcinoma (MDSCC), and poorly differentiated squamous cell carcinoma (PDSCC). Two observers independently assessed TATE using Sirius red stain, with the average eosinophil count evaluated in 10 fields under the ×40 objective lens. Statistical analysis involved the Student t-test, One-way ANOVA, and chi-square test. The study encompassed 70 OSCC samples and corresponding healthy tissue, with OSCC predominantly found in the tongue, representing 61.4% of cases. The total eosinophil count per high-power field (HPF10) was significantly higher in oral squamous cell carcinoma (OSCC) compared to healthy tissue. Furthermore, the mean TATE score was found to be considerably elevated in OSCC tissue. However, a one-way analysis of variance (ANOVA) revealed a statistically non-significant association between different grades of OSCC and eosinophil counts. However, the chi-square test did not indicate a significant association between eosinophil count and gender or age group. The present study underscores the heightened eosinophil count observed in cancerous tissues compared to healthy tissues. However, the variability in eosinophil counts across distinct OSCC grades remains ambiguous. Further investigation is recommended to delve into the infiltration of eosinophils in solid tumors and their potential role in predicting malignancies, particularly in OSCC.
肿瘤相关组织嗜酸性粒细胞增多症（TATE）与包括口腔鳞状细胞癌在内的多种肿瘤有关。然而，TATE在这些情况下的确切作用仍有待充分阐明。本研究旨在调查口腔鳞状细胞癌（OSCC）中肿瘤相关组织嗜酸性粒细胞增多症的存在情况及其与组织病理学分级的相关性。2016年至2020年期间共收集了70份OSCC组织样本进行检查。这些样本包括60例先前诊断的OSCC病例，分为高分化鳞状细胞癌（WDSCC）、中分化鳞状细胞癌（MDSCC）和低分化鳞状细胞癌（PDSCC）。两名观察者使用天狼星红染色法独立评估TATE，在×40物镜下的10个视野中评估嗜酸性粒细胞的平均计数。统计分析采用Student t检验、单因素方差分析和卡方检验。该研究涵盖了70份OSCC样本和相应的健康组织，OSCC主要发生在舌部，占病例的61.4%。与健康组织相比，口腔鳞状细胞癌（OSCC）中每高倍视野（HPF10）的嗜酸性粒细胞总数显著更高。此外，发现OSCC组织中的平均TATE评分显著升高。然而，单因素方差分析（ANOVA）显示不同分级的OSCC与嗜酸性粒细胞计数之间在统计学上无显著关联。然而，卡方检验未表明嗜酸性粒细胞计数与性别或年龄组之间存在显著关联。本研究强调了与健康组织相比，癌组织中观察到的嗜酸性粒细胞计数增加。然而，不同OSCC分级中嗜酸性粒细胞计数的变异性仍不明确。建议进一步研究以深入探讨嗜酸性粒细胞在实体瘤中的浸润情况及其在预测恶性肿瘤，特别是在OSCC中的潜在作用。
