University of New Mexico, Department of Physics and Astronomy, Albuquerque, New Mexico, United States.
University of New Mexico, Comprehensive Cancer Center, Albuquerque, New Mexico, United States.
J Biomed Opt. 2024 Aug;29(8):080501. doi: 10.1117/1.JBO.29.8.080501. Epub 2024 Aug 8.
The technique of remote focusing (RF) has attracted considerable attention among microscopists due to its ability to quickly adjust focus across different planes, thus facilitating quicker volumetric imaging. However, the difficulty in changing objectives to align with a matching objective in a remote setting while upholding key requirements remains a challenge.
We aim to propose a customized yet straightforward technique to align multiple objectives with a remote objective, employing an identical set of optical elements to ensure meeting the criteria of remote focusing.
We propose a simple optical approach for aligning multiple objectives with a singular remote objective to achieve a perfect imaging system. This method utilizes readily accessible, commercial optical components to meet the fundamental requirements of remote focusing.
Our experimental observations indicate that the proposed RF technique offers at least comparable, if not superior, performance over a significant axial depth compared with the conventional RF technique based on commercial lenses while offering the flexibility to switch the objective for multi-scale imaging.
The proposed technique addresses various microscopy challenges, particularly in the realm of multi-resolution imaging. We have experimentally demonstrated the efficacy of this technique by capturing images of focal volumes generated by two distinct objectives in a water medium.
远程聚焦（RF）技术因其能够快速调整不同平面的焦点，从而促进更快的体积成像，引起了显微镜专家的极大关注。然而，在远程环境中，要改变物镜以与远程物镜匹配，并保持关键要求，仍然具有挑战性。
我们旨在提出一种定制的、简单的技术，以便在远程环境中用一个单一的远程物镜来对准多个物镜，使用相同的一组光学元件来确保符合远程聚焦的标准。
我们提出了一种简单的光学方法，用于将多个物镜与单个远程物镜对准，以实现完美的成像系统。该方法利用现成的商业光学元件来满足远程聚焦的基本要求。
我们的实验观察表明，与基于商业透镜的传统 RF 技术相比，所提出的 RF 技术在显著的轴向深度上提供了至少可比的性能，如果不是更好的性能，同时提供了切换物镜进行多尺度成像的灵活性。
所提出的技术解决了各种显微镜挑战，特别是在多分辨率成像领域。我们通过在水介质中拍摄两个不同物镜产生的焦点体积的图像，实验证明了该技术的有效性。
