Delesalle Véronique A, Ankeriasniemi Ruusu E, Lewis Colin M, Mody Jehan M, Roy Abigail M, Sarvis Ward A, Vo Duy D, Walsh Allison E, Zappia Rose J
Department of Biology, Gettysburg College, Gettysburg, Pennsylvania, USA.
Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
Phage (New Rochelle). 2024 Jun 21;5(2):84-90. doi: 10.1089/phage.2024.0004. eCollection 2024 Jun.
As part of a large science education effort, bacteriophages that lyse mc155 continue to be discovered.
Phages were isolated from soil samples from urban sites in the Northeastern United States. Their genomes were sequenced, assembled, and bioinformatically compared.
Three lytic siphoviruses belonging to subcluster B3 with high similarity to each other and other B3 mycobacteriophages were isolated. These phages contain double-stranded DNA genomes (68,754 to 69,495 bp) with high GC content (67.4-67.5%) and 102-104 putative protein coding genes. Notable features include a HicA-like toxin and 33 genes exclusive to subcluster B3. One phage had an intein in its terminase sequence.
Genomic analyses of these phages provide insights into genome evolution and horizontal gene transfer (HGT). The networks for HGT are apparently vast and gene specific. Interestingly, a number of genes are found in both B3 and DR phages.
作为一项大型科学教育工作的一部分，能够裂解mc155的噬菌体仍在不断被发现。
从美国东北部城市地区的土壤样本中分离噬菌体。对它们的基因组进行测序、组装，并进行生物信息学比较。
分离出了三种属于B3亚群的裂解性长尾噬菌体，它们彼此之间以及与其他B3分枝杆菌噬菌体具有高度相似性。这些噬菌体含有双链DNA基因组（68,754至69,495碱基对），GC含量高（67.4 - 67.5%），有102 - 104个推定的蛋白质编码基因。显著特征包括一个类HicA毒素和33个B3亚群特有的基因。一种噬菌体在其末端酶序列中有一个内含肽。
这些噬菌体的基因组分析为基因组进化和水平基因转移（HGT）提供了见解。HGT网络显然很庞大且具有基因特异性。有趣的是，在B3和DR噬菌体中都发现了一些基因。
