两种针对肠道致病性/肠外致病性杂交菌株的新型噬菌体
Two Novel Bacteriophage Species Against Hybrid Intestinal Pathogenic /Extraintestinal Pathogenic Strains.
作者信息
Imklin Napakhwan, Sriprasong Pattaraporn, Thanantong Narut, Lekcharoensuk Porntippa, Nasanit Rujikan
机构信息
Department of Biotechnology, Faculty of Engineering and Industrial Technology, Silpakorn University, Nakhon Pathom, Thailand.
Department of Farm Resources and Production Medicine, Faculty of Veterinary Medicine, Kasetsart University, Nakhon Pathom, Thailand.
出版信息
Phage (New Rochelle). 2024 Jun 21;5(2):107-116. doi: 10.1089/phage.2023.0020. eCollection 2024 Jun.
BACKGROUND
Colibacillosis caused by is one of the main problems in the swine industry. In addition, the emergence of antimicrobial resistance and the combination of virulence genes among pathotypes have led to the emergence of more virulent pathogenic strains. Phage therapy has become a promising approach to address these issues.
MATERIALS AND METHODS
Virulence genes for intestinal pathogenic (IPEC) and extraintestinal pathogenic (ExPEC) were investigated in pathogenic isolated from pigs. In addition, two potential phages, vB_EcoM-RPN187 and vB_EcoM-RPN226, isolated in our previous study, were further characterized in this study.
RESULTS
Both phages were lytic and were highly effective at 20-37°C. Interestingly, they infected the hybrid IPEC/ExPEC strains. vB_EcoM-RPN187 and vB_EcoM-RPN226 possess 167 kbp of linear double-stranded DNA without virulence or antibiotic resistance genes and may be classified as new phage species in the genera and , respectively.
CONCLUSION
Both phages could be promising candidates for phage therapy against pathogenic .
背景
由大肠杆菌引起的大肠杆菌病是养猪业的主要问题之一。此外，抗菌药物耐药性的出现以及致病型别之间毒力基因的组合导致了更具毒性的致病性大肠杆菌菌株的出现。噬菌体疗法已成为解决这些问题的一种有前景的方法。
材料与方法
对从猪分离出的致病性大肠杆菌中的肠道致病性大肠杆菌（IPEC）和肠外致病性大肠杆菌（ExPEC）的毒力基因进行了研究。此外，在本研究中对我们先前研究中分离出的两种潜在噬菌体vB_EcoM-RPN187和vB_EcoM-RPN226进行了进一步表征。
结果
两种噬菌体均具有裂解性，在20-37°C时高效。有趣的是，它们感染了混合型IPEC/ExPEC菌株。vB_EcoM-RPN187和vB_EcoM-RPN226分别拥有167 kbp的线性双链DNA，无毒力或抗生素抗性基因，可能分别被归类为T4样噬菌体属和T5样噬菌体属中的新噬菌体物种。
结论
两种噬菌体都可能是针对致病性大肠杆菌进行噬菌体疗法的有前景的候选者。
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