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[吡喹酮治疗神经囊尾蚴病时脑脊液和血清的ELISA(IgG和IgM):与补体结合反应和免疫荧光法的比较]

[ELISA (IgG and IgM) of the CSF and serum in neurocysticercosis under treatment with praziquantel: comparison with complement fixation reactions and immunofluorescence].

作者信息

Livramento J A, Costa J M, Machado L R, Nóbrega J P, Spina-França A

出版信息

Arq Neuropsiquiatr. 1985 Sep;43(3):267-74. doi: 10.1590/s0004-282x1985000300005.

DOI:10.1590/s0004-282x1985000300005
PMID:3911928
Abstract

Cerebrospinal fluid (CSF) and serum samples of 10 patients with neurocysticercosis under treatment by praziquantel were studied in four occasions: before treatment (day 0) and during treatment on three occasions (days 1, 7 and 21). The immunological reaction analyzed were: ELISA-G and ELISA-M; immunofluorescence-G and immunofluorescence-M; complement fixation. The changes and the variability of results observed through these reactions in this study bring new data that confirm neuroimmunological findings already described in the disease. Acute phase response was found in CSF in 6 cases and only in one case in serum by ELISA-M and immunofluorescence-M. ELISA-G in CSF and serum showed a better sensitivity than the other reactions for diagnosis and for follow-up. Emphasis is given to the importance of the simultaneous realization of several immunological reactions in CSF and serum in patients with neurocysticercosis.

摘要

对10例接受吡喹酮治疗的神经囊尾蚴病患者的脑脊液(CSF)和血清样本进行了4次研究:治疗前(第0天)和治疗期间的3次(第1、7和21天)。分析的免疫反应包括:ELISA - G和ELISA - M;免疫荧光 - G和免疫荧光 - M;补体结合试验。通过本研究中这些反应观察到的结果变化和变异性带来了新的数据,证实了该疾病中已描述的神经免疫学发现。通过ELISA - M和免疫荧光 - M在6例患者的脑脊液中发现了急性期反应,而血清中仅在1例患者中发现。脑脊液和血清中的ELISA - G在诊断和随访方面显示出比其他反应更好的敏感性。强调了对神经囊尾蚴病患者同时进行脑脊液和血清中多种免疫反应检测的重要性。

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[ELISA (IgG and IgM) of the CSF and serum in neurocysticercosis under treatment with praziquantel: comparison with complement fixation reactions and immunofluorescence].[吡喹酮治疗神经囊尾蚴病时脑脊液和血清的ELISA(IgG和IgM):与补体结合反应和免疫荧光法的比较]
Arq Neuropsiquiatr. 1985 Sep;43(3):267-74. doi: 10.1590/s0004-282x1985000300005.
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Characterization by enzyme-linked immunosorbent assay of the humoral immune response in patients with neurocysticercosis and its application in immunodiagnosis.酶联免疫吸附测定法对神经囊尾蚴病患者体液免疫反应的表征及其在免疫诊断中的应用。
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