[采用免疫酶联试验（ELISA）检测神经囊尾蚴病患者脑脊液中抗猪囊尾蚴IgM抗体]

[Detection by the immunoenzymatic test ELISA of IgM anti-Cysticercus cellulosae antibodies in the cerebrospinal fluid in neurocysticercosis].

作者信息

Costa J M, Mineo R, Livramento J A, Camargo M E

出版信息

Arq Neuropsiquiatr. 1985 Mar;43(1):22-8. doi: 10.1590/s0004-282x1985000100004.

DOI:10.1590/s0004-282x1985000100004

Abstract

IgM antibodies against Cysticercus cellulosae in the cerebrospinal fluid (CSF) was demonstrated by ELISA immunoenzymatic assay in neurocysticercosis. CSF samples of 41 patients were analyzed for this purpose. Diagnosis was neurocysticercosis in 26 and neurosyphilis in 5; abnormalities were not registered in the other 10 cases. Neurosyphilis samples and no-abnormalities samples were considered as control groups. ELISA IgM assay for cysticercosis was negative in all CSF samples of control groups and it was positive in 12 of the 26 CSF samples of the neurocysticercosis group (46.2%). Titers ranged from 4 till 32. Positive results were no more obtained after previous treatment of CSF samples by 2-mercaptoethanol. ELISA IgM and IgG titers were compared. IgM titers wee higher than IgG titers in two cases. Results obtained were compared to those found through complement fixation, immunofluorescence and hemagglutination tests for the diagnosis of neurocysticercosis.

摘要

通过酶联免疫吸附测定（ELISA）在神经囊尾蚴病患者的脑脊液（CSF）中检测到抗猪囊尾蚴的IgM抗体。为此分析了41例患者的脑脊液样本。诊断为神经囊尾蚴病的有26例，神经梅毒的有5例；其他10例未发现异常。将神经梅毒样本和无异常样本作为对照组。对照组所有脑脊液样本中囊尾蚴病的ELISA IgM检测均为阴性，而神经囊尾蚴病组26例脑脊液样本中有12例呈阳性（46.2%）。滴度范围为4至32。用2-巯基乙醇预处理脑脊液样本后未再获得阳性结果。比较了ELISA IgM和IgG滴度。有2例IgM滴度高于IgG滴度。将所得结果与通过补体结合、免疫荧光和血凝试验诊断神经囊尾蚴病的结果进行了比较。