Jansson P E, Lönngren J, Widmalm G, Leontein K, Slettengren K, Svenson S B, Wrangsell G, Dell A, Tiller P R
Carbohydr Res. 1985 Dec 15;145(1):59-66. doi: 10.1016/s0008-6215(00)90412-9.
The O-antigen polysaccharides of Klebsiella serotype O5 and Escherichia coli serotype O8 are serologically very similar or identical. The structures of these two polysaccharides have now been re-investigated. N.m.r. spectroscopy, chromium trioxide oxidation, hydrolysis with a specific phage enzyme, and f.a.b. mass spectrometry were the principal methods used. It is concluded that the O-antigen has the following structure, in which D-Man3Me is 3-O-methyl-D-mannose and n is approximately 10. (Formula: see text) Biosynthetic studies indicate that these antigens are synthesised by addition of D-mannopyranosyl groups to the "non-reducing" end of the mannan chain, and it seems possible that addition of a 3-O-methyl-D-mannopyranosyl group involves termination.
肺炎克雷伯菌O5血清型和大肠杆菌O8血清型的O抗原多糖在血清学上非常相似或相同。现已对这两种多糖的结构进行了重新研究。核磁共振光谱、三氧化铬氧化、用特定噬菌体酶水解以及快原子轰击质谱是主要使用的方法。得出的结论是，O抗原具有以下结构，其中D-Man3Me是3-O-甲基-D-甘露糖，n约为10。（公式：见正文）生物合成研究表明，这些抗原是通过将D-甘露吡喃糖基添加到甘露聚糖链的“非还原”末端而合成的，并且添加一个3-O-甲基-D-甘露吡喃糖基似乎可能涉及终止反应。
