Shen Yi, Huang Derun, Zhang Zhenhua, Fan Yeyang, Sheng Zhonghua, Zhuang Jieyun, Shen Bo, Zhu Yujun
College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310012, China.
State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China.
Plants (Basel). 2024 Jul 25;13(15):2054. doi: 10.3390/plants13152054.
Grain size is a primary determinant of grain weight, which is one of the three essential components of rice grain yield. Mining the genes that control grain size plays an important role in analyzing the regulation mechanism of grain size and improving grain appearance quality. In this study, two closely linked quantitative trait loci (QTL) controlling grain size, were dissected and fine-mapped in a 515.6-kb region on the long arm of chromosome 10 by using six near isogenic line populations. One of them, , which controlled 1000 grain weight (TGW) and grain width (GW), was delimited into a 68.1-kb region containing 14 annotated genes. The Teqing allele increased TGW and GW by 0.17 g and 0.011 mm with the of 12.7% and 11.8%, respectively. The other one, , which controlled grain length (GL), was delimited into a 137.3-kb region containing 22 annotated genes. The IRBB52 allele increased GL by 0.018 mm with the of 6.8%. Identification of these two QTL provides candidate regions for cloning of grain size genes.
粒重是稻谷产量三个基本构成要素之一，而粒大小是决定粒重的主要因素。挖掘控制粒大小的基因对于分析粒大小调控机制及改善粒外观品质具有重要作用。本研究利用6个近等基因系群体，在第10号染色体长臂上一个515.6 kb的区域内，对两个紧密连锁的控制粒大小的数量性状基因座（QTL）进行了解析和精细定位。其中一个QTL，控制千粒重（TGW）和粒宽（GW），被定位到一个68.1 kb的区域，该区域包含14个注释基因。特青等位基因使TGW和GW分别增加0.17 g和0.011 mm，增效分别为12.7%和11.8%。另一个QTL，控制粒长（GL），被定位到一个137.3 kb的区域，该区域包含22个注释基因。IRBB52等位基因使GL增加0.018 mm，增效为6.8%。这两个QTL的鉴定为粒大小基因的克隆提供了候选区域。
