Sokolov Viktor Andreevich, Panikhin Pavel Alexandrovich, Plotnikov Kirill Olegovich, Chepurnov Grigory Yurievich, Blinov Alexander Genadievich
Laboratory of Plant Cytology and Apomixis, Institute of Molecular and Cell Biology, Siberian Branch of the Russian Academy of Sciences, Academician Lavrentyev Avenue, 8/2, 630090 Novosibirsk, Russia.
Laboratory of Food Plants Introduction, Central Siberian Botanical Garden, Siberian Branch of the Russian Academy of Sciences, Zolotodolinskaya Street, 101, 630090 Novosibirsk, Russia.
Plants (Basel). 2024 Aug 1;13(15):2138. doi: 10.3390/plants13152138.
Maize ( L.) is one of the most demanded grain crops in the world. Currently, production has exceeded one billion tons and is increasing by 3-5% annually. Such growth is due to the genetic potential of the crop and the use of heterosis F1 hybrids in production. However, the need to produce first-generation seed annually poses significant challenges and is an economically costly technology. A solution to this problem may be the transfer of the asexual (apomictic) mode of reproduction to maize from its wild relative, eastern gamagrass ( L.). In this work, we report the production of 56-chromosome apomictic hybrids of maize ( L.) with eastern gamagrass ( L.) with restored anther fertility. The mode of reproduction of the plant was confirmed by counting chromosomes and sequencing the nuclear gene () and chloroplast tRNA-Leu () gene. These apomictic hybrids had karyotypes of 2n = 56 = [(10Zm(573MB) + 36Td) + 10Zm(611CB)] and 2n = 56 = [(10Zm(611CB) + 36Td) + 10Zm(611CB)]. The resulting hybrids can be widely used as a fodder crop.
玉米(L.)是世界上需求最大的谷类作物之一。目前,其产量已超过10亿吨,且每年以3%至5%的速度增长。这种增长归因于该作物的遗传潜力以及生产中对杂种优势F1代杂交种的利用。然而,每年都需要生产第一代种子带来了重大挑战,并且是一项经济成本高昂的技术。解决这个问题的一个办法可能是将无性(无融合生殖)繁殖方式从其野生近缘种——东部类蜀黍(L.)转移到玉米上。在这项研究中,我们报告了具有恢复花药育性的玉米(L.)与东部类蜀黍(L.)的56条染色体无融合生殖杂种的产生。通过对染色体计数以及对核基因()和叶绿体tRNA - Leu()基因进行测序,确认了该植物的繁殖方式。这些无融合生殖杂种的核型为2n = 56 = [(10条玉米染色体(573兆碱基)+ 36条类蜀黍染色体)+ 10条玉米染色体(611兆碱基对)]和2n = 56 = [(10条玉米染色体(611兆碱基对)+ 36条类蜀黍染色体)+ 10条玉米染色体(611兆碱基对)]。所得到的杂种可广泛用作饲料作物。