Department of Science in Korean Medicine, Graduate School, Kyung Hee University, Seoul 02447, Republic of Korea.
Department of Korean Medicine, Graduate School, Kyung Hee University, Seoul 02447, Republic of Korea.
Int J Mol Sci. 2024 Jul 25;25(15):8101. doi: 10.3390/ijms25158101.
(TJ) fruit, is a herb that is traditionally used for erectile dysfunction (ED). Given the shared mechanisms of ED and hypertension through vascular smooth muscle, we hypothesized that TJ would be effective in vasodilation and blood pressure reduction. This study confirmed the authenticity of TJ samples via DNA barcoding and quantified the main active compound, torilin, using HPLC. TJ was extracted with distilled water (TJW) and 50% ethanol (TJE), yielding torilin contents of 0.35 ± 0.01% and 2.84 ± 0.02%, respectively. Ex vivo tests on thoracic aortic rings from Sprague-Dawley rats showed that TJE (3-300 µg/mL) induced endothelium-independent, concentration-dependent vasodilation, unlike TJW. Torilin caused concentration-dependent relaxation with an EC of 210 ± 1.07 µM. TJE's effects were blocked by a voltage-dependent K channel blocker and alleviated contractions induced by CaCl and angiotensin II. TJE inhibited vascular contraction induced by phenylephrine or KCl via extracellular CaCl and enhanced inhibition with nifedipine, indicating involvement of voltage-dependent and receptor-operated Ca channels. Oral administration of TJE (1000 mg/kg) significantly reduced blood pressure in spontaneously hypertensive rats. These findings suggest TJ extract's potential for hypertension treatment through vasorelaxant mechanisms, though further research is needed to confirm its efficacy and safety.
（TJ）果，是一种传统上用于治疗勃起功能障碍（ED）的草药。鉴于 ED 和高血压通过血管平滑肌的共同机制，我们假设 TJ 对血管扩张和降低血压会有效。本研究通过 DNA 条形码确认了 TJ 样本的真实性，并使用 HPLC 定量了主要的活性化合物，torilin。TJ 用蒸馏水（TJW）和 50%乙醇（TJE）提取，分别得到 0.35±0.01%和 2.84±0.02%的 torilin 含量。对 Sprague-Dawley 大鼠胸主动脉环的离体试验表明，TJE（3-300μg/mL）诱导非内皮依赖性、浓度依赖性的血管舒张，与 TJW 不同。Torilin 引起浓度依赖性松弛，EC 为 210±1.07μM。TJE 的作用被电压依赖性 K 通道阻滞剂阻断，并减轻了由 CaCl 和血管紧张素 II 引起的收缩。TJE 通过细胞外 CaCl 抑制由苯肾上腺素或 KCl 引起的血管收缩，并增强了硝苯地平的抑制作用，表明涉及电压依赖性和受体操作的 Ca 通道。TJE（1000mg/kg）的口服给药显著降低了自发性高血压大鼠的血压。这些发现表明 TJ 提取物通过血管舒张机制治疗高血压的潜力，但需要进一步研究来确认其疗效和安全性。
