National Immunological Laboratory of Traditional Chinese Medicines, Department of Infection, Affiliated Hospital of Youjiang Medical College of Nationalities, Baise 533000, China.
National Engineering Research Center of Southwest Endangered Medicinal Resource Development, Guangxi Botanical Garden of Medicinal Plants, Nanning, Guangxi 530023, China.
Int J Biol Macromol. 2024 Oct;278(Pt 1):134599. doi: 10.1016/j.ijbiomac.2024.134599. Epub 2024 Aug 8.
AIDS is a serious disease with impaired immune function caused by human immunodeficiency virus (HIV) infection. The treatment of AIDS has always been the focus of global scientific research, and Tat protein is a key regulatory protein in the process of HIV infection. Its high expression is closely related to virus replication, disease progression, etc. The aim of this study is to explore the molecular mechanism of regulating Tat protein expression by using network pharmacology based traditional Chinese medicine for calming the liver and detoxifying. 129 AIDS patients were enrolled in the study and randomly divided into HAART combined with PGJDP treatment and HAART alone treatment groups. The virological response rate, immunological response status (CD4 + T cell level, CD4/CD8) and incidence of abnormal liver function were observed before and 48 weeks after treatment. Using the TCMSP database to obtain the chemical components and targets of the main traditional Chinese medicine components in PGJDP, clinical results indicate that the combination of HAART and PGJDP treatment can improve the virological response rate (P > 0.05); Increase the number of CD4 + T lymphocytes (P > 0.05); Significantly increased CD4/CD8 ratio (P < 0.01); Simultaneously, it significantly reduced the incidence of liver dysfunction (P < 0.01). After screening and analysis, the Chinese herbal medicine for calming liver and detoxifying has the potential to significantly regulate the expression of Tat protein. These Chinese herbal compounds can reduce the expression of Tat protein by affecting key pathways and regulating the expression of related genes, which has potential therapeutic effects on the treatment of AIDS.
艾滋病是一种严重的疾病，其免疫功能受损是由人类免疫缺陷病毒（HIV）感染引起的。艾滋病的治疗一直是全球科学研究的重点，而 Tat 蛋白是 HIV 感染过程中的关键调节蛋白。其高表达与病毒复制、疾病进展等密切相关。本研究旨在探讨基于网络药理学的疏肝解毒中药调节 Tat 蛋白表达的分子机制。将 129 例艾滋病患者纳入研究，并随机分为 HAART 联合 PGJDP 治疗和 HAART 单独治疗组。观察治疗前和治疗后 48 周的病毒学应答率、免疫应答状态（CD4+T 细胞水平、CD4/CD8）和肝功能异常发生率。利用 TCMSP 数据库获取 PGJDP 中主要中药成分的化学成分和靶点，临床结果表明，HAART 联合 PGJDP 治疗可提高病毒学应答率（P>0.05）；增加 CD4+T 淋巴细胞数量（P>0.05）；显著提高 CD4/CD8 比值（P<0.01）；同时，显著降低肝功能异常发生率（P<0.01）。经过筛选和分析，疏肝解毒中药具有显著调节 Tat 蛋白表达的潜力。这些中药化合物可以通过影响关键途径和调节相关基因的表达来降低 Tat 蛋白的表达，这对艾滋病的治疗具有潜在的治疗效果。
