Qian Han-Ying, Chen Lu, Zhang Xiao-Man, Qiu Le, Wang Fei, Feng Ting, Shan Jie, Yuan Xun, Chen Xu-Lin
Anhui University of Chinese Medicine Hefei Anhui 230038 China.
Department of Burns, The First Affiliated Hospital of Anhui Medical University Hefei Anhui 230022 China
Nanoscale Adv. 2025 Apr 12. doi: 10.1039/d4na01075b.
Chronic wound treatment poses a substantial challenge to healthcare systems. Certain herbal medicines have demonstrated clinical efficacy in promoting chronic wound healing, yet their therapeutic mechanisms at the molecular level remain elusive due to their complex composition and multifaceted nature. In this study, Ba-Hao ointment (BHO), a sophisticated herbal formulation with diverse ingredients, is selected as a model to precisely investigate its wound-healing mechanism. Network pharmacology analysis and molecular docking simulations reveal that BHO specifically interacts with key wound-healing proteins, including vascular endothelial growth factor A (VEGFA), tumor necrosis factor-α (TNF-α), and interleukin-1β (IL-1β), suggesting its ability to modulate critical biological pathways involved in inflammation and tissue regeneration. Experimental validation further demonstrates that BHO significantly promotes cell proliferation, suppresses bacterial infection, and enhances the expression of essential growth factors such as epidermal growth factor (EGF) and VEGFA in normal human dermal fibroblasts (NHDF), all of which are vital for effective wound healing. studies confirm that BHO accelerates wound closure, reduces inflammation, and promotes the development of well-organized granulation tissue activation of the PI3K-Akt signaling pathway. This study is interesting since it unveils BHO's molecular role in chronic wound healing, furthering herbal medicine development and insights.
慢性伤口治疗对医疗保健系统构成了重大挑战。某些草药已显示出在促进慢性伤口愈合方面的临床疗效，然而，由于其成分复杂且具有多方面性质，其在分子水平的治疗机制仍不明确。在本研究中，八宝软膏（BHO），一种成分多样的复杂草药制剂，被选为模型来精确研究其伤口愈合机制。网络药理学分析和分子对接模拟表明，BHO与关键的伤口愈合蛋白特异性相互作用，包括血管内皮生长因子A（VEGFA）、肿瘤坏死因子-α（TNF-α）和白细胞介素-1β（IL-1β），表明其能够调节参与炎症和组织再生的关键生物学途径。实验验证进一步表明，BHO显著促进细胞增殖、抑制细菌感染，并增强正常人皮肤成纤维细胞（NHDF）中表皮生长因子（EGF）和VEGFA等重要生长因子的表达，所有这些对有效的伤口愈合都至关重要。研究证实，BHO通过激活PI3K-Akt信号通路加速伤口闭合、减轻炎症并促进组织良好的肉芽组织形成。这项研究很有趣，因为它揭示了BHO在慢性伤口愈合中的分子作用，推动了草药医学的发展和认知。
