Lanka E, Fürste J P, Yakobson E, Guiney D G
Plasmid. 1985 Nov;14(3):217-23. doi: 10.1016/0147-619x(85)90005-8.
Genes specifying DNA primases (pri) are common in all IncP plasmids examined so far. These plasmids suppress the thermosensitive character of the Escherichia coli dnaG3 mutation. The mechanism of suppression appears to be identical to that known for RP4 and IncI alpha plasmids. The DNA primases of both these plasmid types can substitute for the dnaG protein in chromosomal DNA replication. The pri genes of the alpha and beta subgroup of IncP plasmids are related to each other as judged from Southern hybridization and immunological data. Extensive DNA and protein sequence homology has been detected although the gene products of the alpha and beta subgroups exhibit substantial differences in size. The arrangement of overlapping genes at the pri locus of IncP alpha plasmids also appears to be present in the IncP beta group.
到目前为止，在所有已检测的IncP质粒中，编码DNA引发酶（pri）的基因很常见。这些质粒可抑制大肠杆菌dnaG3突变的温度敏感特性。抑制机制似乎与已知的RP4和IncIα质粒相同。这两种质粒类型的DNA引发酶都可以替代染色体DNA复制中的dnaG蛋白。从Southern杂交和免疫学数据判断，IncP质粒α和β亚组的pri基因彼此相关。尽管α和β亚组的基因产物在大小上存在显著差异，但已检测到广泛的DNA和蛋白质序列同源性。IncPα质粒pri位点重叠基因的排列似乎在IncPβ组中也存在。
