Key Laboratory of Neuroregeneration of Jiangsu Province and Ministry of Education, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, 226001, Jiangsu, China.
Key Laboratory of Neuroregeneration of Jiangsu Province and Ministry of Education, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, 226001, Jiangsu, China.
Gene Expr Patterns. 2024 Sep;53:119374. doi: 10.1016/j.gep.2024.119374. Epub 2024 Aug 10.
Wnt signal is crucial to correctly regenerate tissues along the original axis in many animals. Lizards are able to regenerate their tails spontaneously, while the anterior-posterior axis information required for the successful regeneration is still elusive. In this study, we investigated the expression pattern of Wnt ligands and HOX genes during regeneration. The results of in situ hybridization revealed that Wnt6 and Wnt10A mRNA levels are higher in wound epithelium (WE) than that in blastema during regeneration. In addition, we showed that Wnt agonist positively regulated the expression of HOXA13 in cultured blastema cells, while did not show similar effect on that of HOXB13, HOXC13 and HOXD13. Finally, we found that HOXA13 showed a gradient level along the anterior-posterior axis of regenerated blastema, with higher level at the caudal end. These data proposed that Wnt6, Wnt10A and HOXA13 might play an important role in establishing distal position for regeneration.
Wnt 信号对于许多动物沿原始轴正确地再生组织至关重要。蜥蜴能够自发地再生尾巴，而成功再生所需的前后轴信息仍然难以捉摸。在这项研究中，我们研究了 Wnt 配体和 HOX 基因在再生过程中的表达模式。原位杂交的结果表明，Wnt6 和 Wnt10A mRNA 的水平在再生过程中的伤口上皮（WE）中高于芽基。此外，我们表明 Wnt 激动剂正向调节培养的芽基细胞中 HOXA13 的表达，而对 HOXB13、HOXC13 和 HOXD13 没有类似的影响。最后，我们发现 HOXA13 在再生芽基的前后轴上呈现出梯度水平，在尾部更高。这些数据表明 Wnt6、Wnt10A 和 HOXA13 可能在为再生建立远端位置方面发挥重要作用。
