Phadke Kruttika S, Higdon Nathaniel B A, Bellaire Bryan H
Veterinary Microbiology and Preventive Medicine, Iowa State University, Ames 50011, USA.
Interdepartmental Microbiology Graduate Program, Iowa State University, Ames 50011, USA.
Access Microbiol. 2024 Jul 22;6(7). doi: 10.1099/acmi.0.000716.v3. eCollection 2024.
A myriad of coronaviruses cause diseases from a common cold to severe lung infections and pneumonia. SARS-CoV-2 was discovered to be the etiologic agent of the Coronavirus pandemic and many laboratory techniques were examined for virus culture and basic and applied research. Understanding the replication kinetics and characterizing the effect the virus has on different cell lines is crucial for developing studies. With the emergence of multiple variants of SARS-CoV-2, a comparison between their infectivity and replication in common cell lines will help give us a clear understanding of their characteristic differences in pathogenicity. In this study we compared the cytopathic effect and replication of Wild-Type (USA/WA1), Omicron (B.1.1.529), and Delta (B.1.617.2) variants on five different cell lines; VeroE6, VeroE6 cells expressing high endogenous ACE2, VeroE6 cells expressing human ACE2 and TMPRSS2, Calu3 cells highly expressing human ACE2 and A549 cells. This data will aid researchers with experimental planning and viral pathogenicity analysis and provide a baseline for testing any future variants.
多种冠状病毒可引发从普通感冒到严重肺部感染和肺炎等疾病。严重急性呼吸综合征冠状病毒2（SARS-CoV-2）被发现是新冠疫情的病原体，人们对许多实验室技术进行了研究，用于病毒培养以及基础和应用研究。了解病毒的复制动力学并明确病毒对不同细胞系的影响对于开展相关研究至关重要。随着SARS-CoV-2多种变体的出现，比较它们在常见细胞系中的感染性和复制情况将有助于我们清楚了解它们在致病性方面的特征差异。在本研究中，我们比较了野生型（美国/华盛顿1型）、奥密克戎（B.1.1.529）和德尔塔（B.1.617.2）变体在五种不同细胞系（非洲绿猴肾细胞系VeroE6、表达高内源性血管紧张素转换酶2（ACE2）的VeroE6细胞、表达人ACE2和跨膜丝氨酸蛋白酶2（TMPRSS2）的VeroE6细胞、高表达人ACE2的Calu3细胞和A549细胞）上的细胞病变效应和复制情况。这些数据将有助于研究人员进行实验规划和病毒致病性分析，并为测试未来的任何变体提供基线。
